Wild Type p53 Protein level and Micronuclei Number formed as a Biomarker Occupational Exposure of Metal in Dental Technicians in Surabaya

Titiek Berniyanti and Retno Pudji Rahayu and Indah Listiana Kriswandini (2018) Wild Type p53 Protein level and Micronuclei Number formed as a Biomarker Occupational Exposure of Metal in Dental Technicians in Surabaya. Asian J. Env. Tech., 2 (1). pp. 44-51. ISSN 2548-6691

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WILD TYPE p53 PROTEIN LEVEL AND MICRONUCLEI NUMBER FORMED AS A BIOMARKER OCCUPATIONAL EXPOSURE OF METAL IN DENTAL TECHNICIANS IN SURABAYA(1).pdf

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Official URL: http://journal.bioremediation-forum.org/index.php/...

Abstract

Exposure of occupational metals (Co,Ni and Cr) lead to the formation of ROS, and cause damage to DNA. ROS also cause G-T transversion mutation of p53 gene, and lead to low expression of the wild type p53. Wild type p53 plays an important role in regulating genes in cell cycle, cell growth, and DNA repair. If DNA repair is hampered there will be chromosomal damage, thereby effecting the formation of micronuclei. The aim of this study was to analyze the effects of PPE, on wild type p53 and the level of micronuclei in dental technicians. This study was observational analytic with cross sectional approach. 40 samples were taken by random sampling. Data retrieved through interviews and observations. Wild type p53 from saliva was analized with indirect ELISA and micronuclei was examined by bucal swab, then analised by HPA and stained by HE. Data was analised by Pearson correlation test. Significant value is p <0.05.There was a significant correlation between the use of PPE with wild type p53 and micronuclei level (r 0.436 and r -0,337). The study proved that the use PPE properly is positively correlated with the wild p53 and p53 correlated with micronuclei in dental technicians.

Item Type: Article
Uncontrolled Keywords: PPE; Dental technician; p53 wild; Micronuclei and DNA Repair
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Dental Public Health
Creators:
CreatorsNIM/NIDN
Titiek BerniyantiNIDN. 0020105802
Retno Pudji RahayuNIDN. 0014115906
Indah Listiana KriswandiniNIDN. 0030106303
Depositing User: Rudy Febiyanto
Date Deposited: 03 Sep 2019 04:05
Last Modified: 19 Dec 2019 02:40
URI: http://repository.unair.ac.id/id/eprint/86004
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