Effect of Green Tea Extract Supplementation in The semen Extender on Post-Thaw Sperm Quality of Simmental Bulls

Suherni Susilowati, NIDN. 0026065905 and Trilas Sardjito, NIDN. 0030055502 and Oky setyo Widodo, NIDN. 0009029101 and Rochmah Kurnijasanti, NIDN. 0019077004 and Wurlina, NIDN. 0018095405 and Erma Safitri, NIDN. 0023076903 and Mustofa Helmi Effendi, NIDN. 0015016209 (2018) Effect of Green Tea Extract Supplementation in The semen Extender on Post-Thaw Sperm Quality of Simmental Bulls. The Philippine Journal of Veterinary Medicine, 55 (2). pp. 127-134. ISSN 0031-7705

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Abstract

The aim of this study was to determine the effects of green tea extract as antioxidant in the extender of frozen semen on the viability, motility, integrity of plasma membrane, percentage of necrosis and apoptosis of spermatozoa. Ejaculates collected from four Simmental bulls were evaluated and placed in 37°C water bath before the cryopreservation process was started. The semen samples were diluted in milk-egg yolk supplemented with green tea extract of 0 (P0) as control, 0.05 (P1), 0.1 (P2), and 0.15 (P3) mg/100 ml extender, respectively. Diluted semen packed in 0.25 ml straws were cryopreserved and stored in liquid nitrogen for four weeks. Cryopreserved semen packed in straws were thawed individually in 37°C water bath for 30 sec then evaluated microscopically. The results showed that P2 had the highest post-thaw sperm viability, motility, and integrity of plasma membrane compared to the other groups (P<0.05). In addition, the percentage of necrosis and apoptosis of sperm were lowest in P2 (P<0.05) compared to the other groups. The results of the study suggest that semen samples diluted in milk-egg yolk supplemented with green tea extract at the rate of 0.1 mg/100 ml extender had the highest semen quality. Thus, green tea extract can potentially act as antioxidant to maintain the quality of post-thaw bull spermatozoa Key words: apoptosis, bull, green tea extract, necrosis, post-thaw, sperm quality

Item Type: Article
Uncontrolled Keywords: apoptosis, bull, green tea extract, necrosis, post-thaw, sperm quality
Subjects: S Agriculture > SF Animal culture > SF191-275 Cattle
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
Divisions: 06. Fakultas Kedokteran Hewan > Ilmu Kedokteran Hewan Dasar
Creators:
CreatorsNIM/NIDN
Suherni Susilowati, NIDN. 0026065905UNSPECIFIED
Trilas Sardjito, NIDN. 0030055502UNSPECIFIED
Oky setyo Widodo, NIDN. 0009029101UNSPECIFIED
Rochmah Kurnijasanti, NIDN. 0019077004santisam99@yahoo.co.id
Wurlina, NIDN. 0018095405UNSPECIFIED
Erma Safitri, NIDN. 0023076903UNSPECIFIED
Mustofa Helmi Effendi, NIDN. 0015016209UNSPECIFIED
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 09 Sep 2019 07:16
Last Modified: 09 Sep 2019 07:16
URI: http://repository.unair.ac.id/id/eprint/86023
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