Influence of crosslinker concentration on the characteristics of erythropoietin-alginate microspheres

Dewi Melani Hariyadi and Tutiek Purwanti and Safira Adilla (2018) Influence of crosslinker concentration on the characteristics of erythropoietin-alginate microspheres. Journal of Pharmacy & Pharmacognosy Research, 6 (4). pp. 250-259. ISSN 0719-4250

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Context: Microspheres have several advantages including protecting proteins from degradation and clearance after administration and produce a long-term therapeutic effect. Erythropoietin as a neuroprotectant agent has protein-like properties, which are susceptible to degradation and have low in vivo bioavailability. Microspheres formulations is one of potential drug delivery system for erythropoietin. Aims: To evaluate the effect of CaCl2 concentration on the characteristics (particle size, morphology, swelling index, and yield) of erythropoietinalginate microspheres. Methods: Erythropoietin-alginate microspheres prepared by ionotropic gelation method with aerosolization technique using sodium alginate as polymer and CaCl2 as crosslinker were dried using freeze drying method with maltodextrin as lyoprotectant. The concentrations of alginate used were 2%, and CaCl2 concentrations were 0.5 M(F1), 0.75 M(F2) and 1M(F3). Results: Results showed smooth and spherical microspheres for all formula with average particle size were 3.23 ± 0.05 μm (F1); 2.99 ± 0.07 μm (F2); and 2.86 ± 0.03 μm (F3). Mass swelling index at 24 h were 1.25 ± 0.10 (F1), 1.18 ± 0.11 (F2), and 1.11 ± 0.10 (F3); at 30 h were 2.00 ± 1.25 (F1), 1.85 ± 0.14 (F2), and 1.72 ± 0.15 (F3) while particle size swelling index at 24 h were 1.15 ± 0.10 (F1), 1.11 ± 0.10 (F2), and 0.97 ± 0.10 (F3); at 30 h were 1.81 ± 0.09 (F1), 1.73 ± 0.15 (F2), and 1.54 ± 0.14 (F3). Respectively yield percentages were 77.76 ± 6.49, 80.01 ± 3.53, and 82.97 ± 4.22. By using One Way ANOVA, it was found that there were significantly differences between three formulas. Conclusions: The particle size of formulas decreased by increasing concentration of CaCl2, whereas no significant difference on swelling index and yield from microspheres with increasing CaCl2 concentration simultaneously.

Item Type: Article
Uncontrolled Keywords: Ca-alginate microspheres; characterization; erythropoietin; ionotropic gelation.
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi
Dewi Melani HariyadiNIDN0026027801
Tutiek PurwantiNIDN0010025705
Safira AdillaNIM051311133086
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 10 Sep 2019 01:12
Last Modified: 16 Jan 2020 02:38
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