Achmad Fuad Hafid and Chie Aoki Utsubo and Adita Ayu Permanasari and Myrna Adianti, NIDN. 0001038207 and Lydia Tumewu and Aty Widyawaruyanti, NIDN. 0026046210 and Sri Puji Astuti Wahyuningsih, NIDN. 0021026604 and Tutik Sri Wahyuni, NIDN. 0025107704 and Maria Inge Lusida and Soetjipto and Hak Hotta (2017) Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus. Asian Pacific Journal of Tropical Biomedicine, 7 (7). pp. 633-639. ISSN 2221-1691

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Abstract

Objective: To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods: Antiviral activities of the crude extracts were examined by cell culture methodusing Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCVactivities was determined by time-of-addition experiments. The effect on HCV RNAreplication and HCV accumulation in cells were analyzed by quantitative reversetranscription-PCR and western blotting, respectively. Results: The dichloromethane (DCM) extract of A.heterophyllus exhibited strong anti HCV activity with an inhibitory concentration (IC) value of (1.5 ± 0.6) mg/mL without obvious toxicity. The DCM extracts from Artocarpus altilis and Artocarpus camansi showed moderate anti-HCV activities with IC 50 50 values being (6.5 ± 0.3) mg/mL and (9.7 ± 1.1)mg/mL, respectively. A time-of-addition studies showed that DCM extractfrom A. heterophyllus inhibited viral entry process though a direct virucidal activity and targeting host cells. HCV RNA replication and HCV protein expression were slightly reduced by the DCM treatment at high concentration. Conclusions: The DCM extract from A. heterophyllus is a good candidate to develop an antiviral agent to prevent HCV grant reinfection following liver transplantation.

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