Sandhy Kartikasari and Sri Puji Astuti Wahyuningsih, NIDN. 0021026604 (2017) Spermatogenic cell count of mice (Mus musculus) after the treatment with polysaccharide-K from Coriolus versiclor extract. Bioscience Research, 14 (3). pp. 1-6. ISSN 1811-9506.

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Abstract

The objectives of this study were to determine the effect of different doses of polysaccharide-K from Coriolus versicolor extract after 35 days of injection on the number of spermatogenic cells in mice (Musmusculus). Twenty eight, 4-8 week old male mice, weight between 20-25 g were used in this study. The animal try were divided into four groups. The first group was the control group (K), treated by giving them aquadest by gavage for 35 days. The other groups (P1,P2, and P3) as the treatment groups, were treated by using three different doses of polysaccharide-K (15, 30, and 60 mg/kg of body weight respectively) injected by gavage for 35 days. Testicular histological slides were prepared to observe the structure of spermatogenic cells. The data of parameters such as the number of spermatogonia cells,spermatocyte cells, and oval spermatid cells were collected. The number of spermatocyte cells and oval spermatid cells was tested using the One Way Anova followed and Duncan. The number of spermatogonia cells was tested using Brown Forsythe. The results showed that spermatogonia and spermatocyte cell numbers were not significantly different in K, P1, P2, and P3 respectively (P>0.5). The number of oval spermatid cells was significantly different (P <0.5). In P1, the number of spermatid cells decreased 7.9% compared to K. The number of oval spermatid cells P2 and P3 was relatively equal to K. The conclusion of this research showed that the polysaccharide-K treatment from C. versicolor extract had no effect on both the number of spermatogonia and spermatocyte cells, but slightly decreased the number of oval spermatids. Polysaccharide-K is still safe to use as a herbal medicine

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