Sri Puji Astuti Wahyuningsih, NIDN. 0021026604 (2013) Aglutination of Mice Sperm in Antibody of 46, 66, and 73 Kda Protein from Rabbit Sperm Membrane. In: ICOWOBAS RAFSS 2013 Proceedings "Science and mathematics as proponents for sustainable future. lbnu Sina Institute for Fundamental Science Studies, pp. 187-191.

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Abstract

This research aimed to investigate the influence provision antibody of 46, 66, and 73 kDa protein from rabbit sperm for mice sperm agglutination. Twenty four male mice of Balb/C strain, 8-10 weeks old and 20-25 g weights were devided into four groups (six mice for control group and 18 mice for treatment group). All of mice were dissected and then taken was epididimys to get the mice mature sperm by flushing method. From the result of flushing using Baker’s buffer, each sperm suspension was taken 100 µl, then added with 10 µg/ml control, 46, 66, and 73 kDa protein’s antibody. Then taken 50 µl of suspension and placed on hollow glass objects, incubated for 20 second before observed using light microscopy at 400x magnification with 3x replicated observations. This research result showed that the average sperm agglutination of control group was 6.20 and treatment group was 18.92(46 kDa), 12.46 (66 kDa), and 19.60 (73 kDa). In addition types of agglutination, there are agglutination between the head with head, head to tail and tail to tail. Conclusions of this research to provision antibody 46, 66, and 73 kDa protein from the rabbit sperm membrane for mice sperm agglutination and different types of antibodies will affect the result sperm agglutination, the most potentially protein was 73 kDa protein.

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