UJI SITOTOKSISITAS EKSTRAK DAUN KEMBANG BULAN (Tithonia diversifolia) TERHADAP LIMBAL MESENCHYMAL STEM CELL KELINCI DENGAN METODE MTT ASSAY

VIDA SEANITA ZAHRO, 061511133037 (2019) UJI SITOTOKSISITAS EKSTRAK DAUN KEMBANG BULAN (Tithonia diversifolia) TERHADAP LIMBAL MESENCHYMAL STEM CELL KELINCI DENGAN METODE MTT ASSAY. Skripsi thesis, Universitas Airlangga.

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Abstract

Kembang bulan leaf extract (Tithonia diversifolia) is known as a herbal plant which have many utilities to cure disease, such as : diabetic, malaria, and other infection. Instead of many usefullness of this plant, Tithonia diversifolia have main biological properties which may induce toxicity of this plant known as sesquiterpene lactones. Aim of this study was to identify the cytotoxicity effect of Kembang bulan leaf extract (Tithonia diversifolia) on limbal mesenchymal stem cell of rabbits using MTT Assay. Sample of this research using limbal mesenchymal stem cell of rabbits. Kembang bulan leaf extract (Tithonia diversifolia) divide into 4 concentration (0,5%, 0,25%, 0,125%, and 0,0625%) and given to limbal mesenchymal stem cell of rabbits in 96 well and would be incubated for 24 hours. The citotoxicity result was obtained using MTT Assay method. Every well would be observed to see cell life level of each concentration. The optical density absorbancy calculated using ELISA reader. Kembang bulan leaf extract with concentration of 0,0625% has the biggest viability with level of existence of limbal mesenchymal stem cell 50% and the others Kembang bulan leaf concentration 0,5%, 0,25%, and 0,125% have less than 50% level of existence limbal mesenchymal stem cell.

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH 231/ 19 Zah u
Uncontrolled Keywords: Tithonia diversifolia, MTT Assay, cytotoxicity, limbal mesenchymal stem cell
Subjects: S Agriculture > SB Plant culture
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsNIM/NIDN
VIDA SEANITA ZAHRO, 061511133037UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorHani Plumeriastuti, NIDN : 0008085905UNSPECIFIED
ContributorNusdianto Triakoso, NIDN : 0005056803UNSPECIFIED
Depositing User: Mrs. Djuwarnik Djuwey
Date Deposited: 18 Sep 2019 06:29
Last Modified: 18 Sep 2019 06:29
URI: http://repository.unair.ac.id/id/eprint/86949
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