Detection of inVA gene of Salmonella From Milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique

Sheila Marty Yanestria, 060810267 and Reina Puspita Rahmaniar, 060810087 and Freshinta Jellia Wibisono, 060313161 and Mustofa Helmi Effendi, NIDN. 0015016209 (2019) Detection of inVA gene of Salmonella From Milkfish (Chanos chanos) at Sidoarjo wet fish market, Indonesia, using polymerase chain reaction technique. Jurnal Veterinary World Departemen of Veterinary Public Health, 12 (1). pp. 170-175. ISSN 2231-0916

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Official URL: http://www.veterinaryworld.org/Vol.12/January-2019...

Abstract

Aim: The study aimed to detect the invA gene in Salmonella isolated from milkfish in the Sidoarjo wet fish market. Materials and Methods: A total of 84 samples were prepared in enrichment media and isolated on the surface of Salmonella Shigella Agar. Salmonella growth produces transparent colonies with blackish color in the middle due to H2S gas formation. Samples were identified as Salmonella based on macroscopic colony morphology. Presumptive Salmonella sp. was put on Bismuth Sulfite Agar media. Salmonella was determined based on the results of the biochemical test that has been carried out using Microbact identification kits from negative gram staining. Results: The results of this study indicate that 32 of 84 samples (38.09%) were Salmonella bacteria. Furthermore, the invA gene detection was carried out using the polymerase chain reaction technique. Electrophoresis results showed four positive samples contained invA gene with a length of 284 bp. Conclusion: Results in this study indicate that contamination of milkfish with Salmonella needs strict hygienic measures to prevent their transmission to human. Keywords: human health, invA gene, milkfish, polymerase chain reaction, Salmonella

Item Type: Article
Uncontrolled Keywords: human health, invA gene, milkfish, polymerase chain reaction, Salmonella
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF780.2-780.7 Veterinary microbiology, bacteriology, virology, mycology
Divisions: 06. Fakultas Kedokteran Hewan > Kesehatan Masyarakat Veteriner
Creators:
CreatorsNIM/NIDN
Sheila Marty Yanestria, 060810267UNSPECIFIED
Reina Puspita Rahmaniar, 060810087UNSPECIFIED
Freshinta Jellia Wibisono, 060313161UNSPECIFIED
Mustofa Helmi Effendi, NIDN. 0015016209UNSPECIFIED
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 01 Oct 2019 08:12
Last Modified: 01 Oct 2019 08:12
URI: http://repository.unair.ac.id/id/eprint/87181
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