Detection of Blatem Gene Of Klebsiella pneumoniae Isolated From Swab Of Food-Producing Animals In East Java

Dr. Mustofa Helmi Effendi, drh., DTAPH., NIDN 0015016209 and I.G. Bintari, - and Dr. Eduardus Bimo Aksono H., drh., M.Kes., NIDN 0020096605 and I.P. Hermawan, - (2018) Detection of Blatem Gene Of Klebsiella pneumoniae Isolated From Swab Of Food-Producing Animals In East Java. Jurnal International Tropical Animal Science Journal, 41 (3). pp. 174-178. ISSN 2615-787X, e-ISSN 2615-790X

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Abstract

Klebsiella pneumoniae is one of 9 bacteria resistance to antibiotics in concern. This research aimed to detect any gene of blaTEM in bacteria of the K. pneumoniae isolated from swab of food-producing animals. In this study, 195 swab samples were taken from 17 sampling locations. Samples obtained were cultivated on selective medium and had several tests including identifcation, antibiotic sensitivity test using Kirby-Bauer method against antibiotics of ampicillin, cefotaxime, amoxicillin, meropenem, and trimetrophrim-sulfamethoxazole, and followed by PCR test for detecting the gene that was responsible for the antibiotic resistances. The results showed that 10 out of 195 samples were found to be K. pneumonia, those were 4 samples originated from dairy cows (SP-S1, SP-S3, SP-B2, SP-G4), 2 samples originated from beef (SPT-K1, SPT-K2), 1 sample originated from chickens (A-W5), and 3 samples originated from fsh (IN-P2, IN-P3, IN-S3). Most of isolates (9/10) were found to be resistant toward amoxicillin. These isolates were SP-S3, SP-B2, SP-G4, SPT-KI, SPT-K2, A-W5, IN-P2, IN-P3, and the IN-S3 and all of them also showed to be positive of blaTEM gene. It could be concluded that most of K. pneumoniae isolates from food animals harbour had Extended Spectrum Beta-Lactamase (ESBL) encoding gene. Keywords: antibiotic resistance, ESBL, food-producing animals, Klebsiella pneumonie, amoxicillin

Item Type: Article
Uncontrolled Keywords: antibiotic resistance, ESBL, food-producing animals, Klebsiella pneumonie, amoxicillin
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF811-909 Veterinary medicine of special organs, regions, and systems
Divisions: 06. Fakultas Kedokteran Hewan > Kesehatan Masyarakat Veteriner
Creators:
CreatorsNIM/NIDN
Dr. Mustofa Helmi Effendi, drh., DTAPH., NIDN 0015016209UNSPECIFIED
I.G. Bintari, -UNSPECIFIED
Dr. Eduardus Bimo Aksono H., drh., M.Kes., NIDN 0020096605UNSPECIFIED
I.P. Hermawan, -UNSPECIFIED
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 27 Sep 2019 08:45
Last Modified: 27 Sep 2019 08:45
URI: http://repository.unair.ac.id/id/eprint/87206
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