Study of Human Amniotic Membrane Mesenchymal Stem Cells Using Gelatin and Alginate as Nontoxic Scaffolds

Nike Hendrijantini, NIDN. 0006105908 and POEDJO HARTONO, NIDN. 8823800016 and Helen Susilowati, . and Cindy K. Hartono, . and Reni P. Daniati, . and FEBRIAN BRAHMANA, NIM011318126304 (2019) Study of Human Amniotic Membrane Mesenchymal Stem Cells Using Gelatin and Alginate as Nontoxic Scaffolds. Advances in Biology and Medicine, 5. pp. 1-7. ISSN 2378-654X

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Perinatal mesenchymal stem cells (MSCs), for example, from amniotic membrane, have advantages over adult sources, such as bone marrow, in terms of ease of availability, cell naivety, tissue stem cell abundance, high capacity of proliferation, and less donor site morbidity, because it does not require invasive procedures. Natural polymer scaffolds, such as gelatin and alginate, are biocompatible. Combination of stem cells from amniotic membrane (hAMSCs) and gelatin or alginate as scaffold can be promising. However, cytotoxicity comparison of gelatin and alginate to hAMSCs has not been widely studied. This study was aimed to compare cytotoxicity of gelatin and alginate on hAMSCs in vitro. Isolation and culture were performed on hAMSCs of the healthy full-term pregnancy. In passage 4, Flow Cytometry CD90, CD105, and CD73 phenotype characterization was done. Colorimetric assay of 3-(4,5-dimethythiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) was performed to measure the cytotoxicity. There were three sample groups: (control group) hAMSCs with alpha-minimum essential medium (α-MEM) solution as control; (gelatin group) hAMSCs with gelatin; (alginate group) hAMSCs with alginate. Each group consisted of 12 samples. Flow cytometry of hAMSCs expressed 28.78% CD90, 36.95% CD105, and 44.41% CD73 surface markers. No sample depicted toxicity in either gelatin or alginate group, and this is indicated by the average percentage of living cells in gelatin 97.26% and in alginate 98.43%. No statistically significant difference (p=0.057) of cytotoxicity was found between gelatin and alginate to hAMSCs. Gelatin and alginate were nontoxic to hAMSCs in vitro.

Item Type: Article
Uncontrolled Keywords: Gelatin; Alginate; Amniotic membrane; Mesenchymal stromal stem cells.
Subjects: R Medicine
R Medicine > RK Dentistry
R Medicine > RK Dentistry > RK641-667 Prosthetic dentistry. Prosthodontics
Divisions: 02. Fakultas Kedokteran Gigi > Prosthodontics
Nike Hendrijantini, NIDN. 0006105908UNSPECIFIED
Helen Susilowati, .UNSPECIFIED
Cindy K. Hartono, .UNSPECIFIED
Reni P. Daniati, .UNSPECIFIED
FEBRIAN BRAHMANA, NIM011318126304NIM011318126304
Depositing User: Rudy Febiyanto
Date Deposited: 26 Sep 2019 03:57
Last Modified: 26 Sep 2019 03:57
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