Comparative in vitro study of the cytotoxicity of gelatine and alginate to human umbilical cord mesenchymal stem cells

Nike Hendrijantini, NIDN. 0006105908 (2019) Comparative in vitro study of the cytotoxicity of gelatine and alginate to human umbilical cord mesenchymal stem cells. Dental Journal (Majalah Kedokteran Gigi), 452 (1). pp. 36-40. ISSN 2442-9740

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Official URL: https://e-journal.unair.ac.id/MKG/article/view/128...

Abstract

Background: Mesenchymal stem cells (MSCs) and scaffold combination constitute a promising approach currently adopted for tissue engineering. Umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are easily obtained and non-invasive. Gelatine and alginate constitute a biocompatible natural polymer scaffold. At present, a cytotoxicity comparison of gelatine and alginate to hUC-MSCs is not widely conducted. Purpose: This study aimed to compare the cytotoxicity of gelatine and alginate in hUC-MSCs in vitro. Methods: Isolation and culture were performed on hUC-MSCs derived from healthy full-term neonates. Flow Cytometry CD90, CD105 and CD73 phenotype characterization was performed in passage 4. 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay was performed to measure the cytotoxicity. The three sample groups were: (T1) hUC-MSCs with α-MEM (alphaminimum essential medium) solution as control; (T2) hUC-MSCs with gelatine; (T3) hUC-MSCs with alginate. Results: Flow cytometry of hUC-MSCs displayed positive CD90, CD105 and CD73 surface markers. Gelatine and alginate had no effect on the viability of hUC-MSCs and no statistically significant difference (p>0.05) of cytotoxicity between gelatine and alginate to hUC-MSCs. Conclusion: Gelatine and alginate proved to be non-toxic to hUC-MSCs in vitro.

Item Type: Article
Uncontrolled Keywords: alginate; gelatine; mesenchymal stem cells; umbilical cord
Subjects: R Medicine
R Medicine > RK Dentistry
R Medicine > RK Dentistry > RK641-667 Prosthetic dentistry. Prosthodontics
Divisions: 02. Fakultas Kedokteran Gigi > Prosthodontics
Creators:
CreatorsNIM/NIDN
Nike Hendrijantini, NIDN. 0006105908UNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 25 Sep 2019 04:16
Last Modified: 25 Sep 2019 04:16
URI: http://repository.unair.ac.id/id/eprint/87296
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