Analysis of Lymphocyte Cell Proliferation and IFN-γ Expression In Saliva of Severe Early Childhood Caries and Caries-Free in Surabaya

Muhammad Luthfi, NIDN. 0006036704 and Aqsa Sjuhada Oki, NIDN. 0003106903 and Retno Indrawati, NIDN. 0012115903 and Nadhia S. Latuamury (2019) Analysis of Lymphocyte Cell Proliferation and IFN-γ Expression In Saliva of Severe Early Childhood Caries and Caries-Free in Surabaya. Malaysian Journal of Medicine and Health Sciences, 15 (3). pp. 8-10. ISSN 2636-9346

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Abstract

Dental caries is a chronic, multifactorial disease and occurs by shifting of biofilm. Shifting of biofilm flora caused by poor oral hygiene, genetic factors, and long-term immune changes lead to an increase in Streptococcus mutans which results in decreased pH and demineralization on tooth surfaces. The body’s immune system serves to defend from foreign invaders. IFN-γ and lymphocytes are innate and adaptive immunity components that are responsible for the occurrence of disease. The role of the immune system becomes important in understanding the mechanisms of disease prevention. The effective function of the body’s immune system is to immediately eradicate the infectious agent from the body. The objective of this study was to analyze the expression of IFN-γ and salivary cell lymphocyte proliferation as an early detection marker of severe early childhood caries. Saliva taken from preschool-aged children 4 to 6 years was divided into two groups, ie heavy caries group with DMFT > 6 and caries-free with dmft = 0, for lymphocyte cell proliferation test using MTT assay and for expression test of IFN-γ using flow cytometry test. There are differences in lymphocyte cell proliferation and IFN-γ expression in the saliva of severe early childhood (S-ECC) caries and caries-free. The increase in lymphocyte proliferation and IFN-γ expression up to 6 hours incubation can be used as the indicator of early detection marker of severe early childhood caries.

Item Type: Article
Additional Information: Summer Crash Course: Environmental and Occupational Health Seminar (SEOH) 2018
Uncontrolled Keywords: Lymphocyte Cell, IFN-γ, Severe early childhood caries (S-ECC), Early detection marker
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Oral Biology
Creators:
CreatorsNIM/NIDN
Muhammad Luthfi, NIDN. 0006036704UNSPECIFIED
Aqsa Sjuhada Oki, NIDN. 0003106903UNSPECIFIED
Retno Indrawati, NIDN. 0012115903UNSPECIFIED
Nadhia S. LatuamuryUNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 30 Sep 2019 07:18
Last Modified: 12 Nov 2019 05:44
URI: http://repository.unair.ac.id/id/eprint/87474
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