Evaluation of Energy Dose and Output Power Optimum of Diode’s Laser of 450 nm and 650 nm in Photoantimicrobial Mechanisms Against Inhibition of C. Albicans Biofilm Cells

S. Dewi Astuty and Suhariningsih, NIDN. 0027065203 and Suryani Dyah Astuti, NIDN. 0008046902 and Afaf Baktir, NIDN. 0014105603 (2018) Evaluation of Energy Dose and Output Power Optimum of Diode’s Laser of 450 nm and 650 nm in Photoantimicrobial Mechanisms Against Inhibition of C. Albicans Biofilm Cells. IOP Conf. Series: Journal of Physics, 979 (2018). pp. 1-8.

[img] Image (ABSTRACT)
C08_Abstract.pdf

Download (598kB)
[img] Text (FULLTEXT)
C08_Karya Ilmiah.pdf

Download (595kB)
[img] Text (REVIEW / VALIDASI)
C08_Reviewer dan Validasi.pdf

Download (3MB)
[img] Text (SIMILARITY)
C08_Similarity.pdf

Download (3MB)
Official URL: https://iopscience.iop.org/article/10.1088/1742-65...

Abstract

Abstract. Photoantimicrobial as a pathogenic microbial inhibitory therapy system such as C. albicans in biofilms forms has been studied in vitro. Mechanisms of inhibiting called inactivating used the absorb principles of a dye agents such as chlorophyll against the photon energy of diode laser which any number of ROS product depend on energy doses of a laser, time of irradiation, concentration and time of incubation the dyes agent. The inactivation profile of C. albicans biofilm cells was observed based on cell viability reduction after photoantimicrobial treatment with or without oxygenation by XTT assay test. Results show that the inhibiting significantly with the time incubation of the dye agents and the oxygen degree inside the sample. The inhibition for oxygenation biofilm’s group 10% lower than without oxygenation biofilm’s group at the maximum of reduction of cell viability occurred in the 3hour incubation group. Optimum of inactivation are 89.6% (without oxygenation) and 94.8% (with oxygenation) after irradiation with 450 nm laser (power output 128.73 at energy dose 86.09 J/cm2), While, by 650 nm laser (power output 164.53 mW at energy dose 92.52 J/cm2) irradiation treatment obtained optimum of inactivation are 89.5% (without oxygenation) and 92.3% (with oxygenation).

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: 08. Fakultas Sains dan Teknologi > Fisika
Creators:
CreatorsEmail
S. Dewi AstutyUNSPECIFIED
Suhariningsih, NIDN. 0027065203UNSPECIFIED
Suryani Dyah Astuti, NIDN. 0008046902UNSPECIFIED
Afaf Baktir, NIDN. 0014105603UNSPECIFIED
Depositing User: Mr Vega Andi Budiman
Date Deposited: 03 Oct 2019 04:40
Last Modified: 03 Oct 2019 04:40
URI: http://repository.unair.ac.id/id/eprint/88108
Sosial Share:

Actions (login required)

View Item View Item