Suryani Dyah Astuti, NIDN. 0008046902 and Ni’matuzahroh and Muhammad Zainuddin, NIDN. 8858370018 and Suhariningsih, NIDN. 0027065203 (2011) Potensi Blue Light Emitting Diode (LED) untuk Fotoinaktivasi Bakteri Staphylococcus aureus dengan Porfirin Endogen. Jurnal Biosains Pascasarjana, 13 (3). pp. 155-163. ISSN 0852-6834

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Abstract

Photodynamic Inactivation (PDI) represents a method for bacteria inactivation by using combination of endogenous porphyrin and light photon. The purpose of this study were to determine the instrument performance of LED light source that had been assembled and to recognize the potency of LED irradiating energy dose for photoinactivation of Staphylococcus aureus bacteria ATCC 6538P with endogenous porphyrin. Performance measurement of LED instrument showed that blue LED had emission peak of 430 nm and red 629 nm with 65 nm and 30 nm bandwidth. The analysis LED irradiating energy dose by anova indicated that interaction of power and irradiating time of blue LED 430 nm decreased the percentage of Staphylococcus aureus colonies. With optimal irradiating of 75 mW/ccm2 power density and 30 minutes time of irradiating (energy density 135 J/cm2), it yielded the percentage decrease of Staphylococcus aureus colonies as many as 75%. Hence, the blue LED instrument has a high potential for photoinactivation to Staphylococcus aureus bacteria.

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