KARAKTERISASI ANTIBODI HASIL INDUKSI PREGNANCY-ASSOCIATED GLYCOPROTEIN (PAG) SERUM DARAH KAMBING PERANAKAN ETAWA BUNTING SEBAGAI KANDIDAT BIOMARKER KIT TES KEBUNTINGAN DINI PENELITIAN EKSPERIMENTAL LABORATORIS

INTAN KUMALA NINGTYAS, 061714153001 (2019) KARAKTERISASI ANTIBODI HASIL INDUKSI PREGNANCY-ASSOCIATED GLYCOPROTEIN (PAG) SERUM DARAH KAMBING PERANAKAN ETAWA BUNTING SEBAGAI KANDIDAT BIOMARKER KIT TES KEBUNTINGAN DINI PENELITIAN EKSPERIMENTAL LABORATORIS. Thesis thesis, Universitas Airlangga.

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Abstract

The aim of this study was to isolate and characterize Pregnancy-Associated Glycoprotein (PAG) derived from blood serum pregnant Etawa crossbreed goat and to prove that PAG can induce humoral immune responses. PAG was isolated from the serum of 3–4 month pregnant Etawa crossbreed goats. The results showed 12 protein bands were identified on the SDS-PAGE examination. PAG isolates with a molecular weight of 55.85 kDa. Antibodies of PAG was found were performed male New Zealand rabbits with PAG isolate. Serum extraction was carried out from the auricular vein. PAG antibody titers analysis using indirect ELISA. PAG can induce a humoral immune response by the formation antibodies. The highest production of PAG antibodies occurring at 7th week bleeding (0.404).

Item Type: Thesis (Thesis)
Additional Information: KKC KK TBR. 05-19 Nin k
Uncontrolled Keywords: Pregnancy-Associated Glycoprotein, pregnant goat, serum, PAG antibodies.
Subjects: S Agriculture > SF Animal culture > SF380-388 Goats
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsNIM/NIDN
INTAN KUMALA NINGTYAS, 061714153001UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorHERRY AGOES HERMADI, NIDN: '0023085904UNSPECIFIED
ContributorTITA DAMAYANTI LESTARI, NIDN: '0002106002UNSPECIFIED
Depositing User: Tatik Poedjijarti
Date Deposited: 03 Oct 2019 05:50
Last Modified: 03 Oct 2019 05:50
URI: http://repository.unair.ac.id/id/eprint/88214
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