PARAMITA PUTRI, NIM011418016306 (2019) PENGARUH MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP PROLIFERASI DAN MORFOLOGI SEL ENDOTEL KORNEA PASCA PAPARAN ULTRASOUND FAKOEMULSIFIKASI (Studi Eksperimental pada Oryctolagus cuniculus). Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Objective : to evaluate LMSC-SM on corneal endothelial cell proliferation and morphology after ultrasound phacoemulsification in rabbit. Material and methods: Twenty four New Zealand white rabbit (24 eyes) were randomly divided into 2 groups. Group A (12 eyes) with right eye as control and whereas Group B (12 eyes) with right eye as treatment. The control group was the group carried out endothelial phaco burn and then given intracameral BSS injection. While the treatment group was the group carried out endothelial phaco burn and then given intracameral LMSC-CM injection. The morphometric parameters was measured on day 3 with specular microscopy and the eyes were enucleated to evaluate cell proliferation shown by Ki-67 expression. Results: this study showed the amount of proliferation cells shown by Ki-67 expression in the treatment group was statistically significant higher than the control group (p = 0.002 , ⍺ < 0.05). While the morphometric parameters of the corneal endothelial cells in the form of CV and hexagonality measured through specular microscopy did not show significant difference between groups. Conclusion: LMSC-CM may promote corneal endothelial wound healing by promoting cell proliferation

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