The Effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm

H. Dwinofanto and Rimayanti, NIDN. 0003126305 and Imam Mustofa, NIDN. 0027046003 and Suherni Susilowati, NIDN. 0026065905 and Tatik Hernawati, NIDN. 0029086005 (2018) The Effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraqi Journal of Veterinary Sciences, 32 (2). pp. 249-252. ISSN 1607-3894

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Abstract

Frozen semen quality is one of many factors that promote successfully of artificial insemination. Preservation at 5ºC is one of the steps of semen freezing to obtain high fertility sperm after added with the extender. This study was aimed to determined the sperm quality (motility, viability, and membrane integrity), MDA level and DNA damage of post-thawed sperm after being preserved at 5ºC for different duration. Bali cattle bull semen collected by artificial vagina. Macroscopic and microscopic evaluation of ejaculate was conducted first. Qualified semen was diluted in Tris Aminomethan-Egg yolk and then devided into two treatment group: preserved at 5ºC for 4 hours (first group) and 22 hours (second group), and continued to processed into frozen semen, and stored overnight. Pre-freezing and post-thawed of semen analysis was conducted based on SNI: 48691:2017 of The National Standardization Agency of Indonesia. Measurement of levels of Malondialdehyde (MDA) was conducted by spectrophotometry at 532 nm wavelength, meanwhile measurement of sperms DNA damage percentage was performed by Toluidine blue staining. The result of showed that quality of post-thawed sperm (motility, viability, and membrane integrity) was higher (P<0.05), and MDA level and DNA damage were lower (P<0.05) in preservation at 5°C for 22 hours compared to those of 4 hours. It could be concluded that preservation at 5°C longer (22 hours) means sperm had longer chance to adapt with the extender, this implies the higher quality and lower lipid peroxidation. Keywords: Bali bull, preservation, MDA, DNA, post-thawed

Item Type: Article
Uncontrolled Keywords: Bali bull, preservation, MDA, DNA, post-thawed
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
Divisions: 06. Fakultas Kedokteran Hewan > Reproduksi Veteriner
Creators:
CreatorsNIM/NIDN
H. DwinofantoUNSPECIFIED
Rimayanti, NIDN. 0003126305UNSPECIFIED
Imam Mustofa, NIDN. 0027046003UNSPECIFIED
Suherni Susilowati, NIDN. 0026065905UNSPECIFIED
Tatik Hernawati, NIDN. 0029086005UNSPECIFIED
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 13 Nov 2019 08:54
Last Modified: 13 Nov 2019 08:54
URI: http://repository.unair.ac.id/id/eprint/89075
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