Induced Monocytes-Derived HSCs (CD34+) with LPS Accelerated Homing Rat Bone Marrow-Mesenchymal Stem Cell (BM-MSCs, CD105) in Injured Pancreas

Fedik Abdul Rantam, NIDN. 0010035907 and Purwati, NIDN. 0025027203 and Budi Setiawan and Sony Wibisono, NIDN. 8827800016 and Ferdiansyah Mahyudin, NIDN. 8890010016 and Joni Wahyuhadi, NIDN. 8885900016 and Edward Mouli and Dwikora Novembri Utomo, NIDN. 8897700016 and Heri Suroto, NIDN. 8802630017 and Candra Bumi (2015) Induced Monocytes-Derived HSCs (CD34+) with LPS Accelerated Homing Rat Bone Marrow-Mesenchymal Stem Cell (BM-MSCs, CD105) in Injured Pancreas. Journal Biomedical Science and Engineering, 08 (05). pp. 333-344. ISSN 1937-687

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Abstract

Investigating the function of combining induced rat monocytes-derived bone marrow-haemopoietic stem cell (rat BM-HSCs) with LPS and rat bone marrow-mesenchymal stem cell (rat BM-MSCs) was to analyze the acceleration of homing process mechanism in injured pancreas. Mononucleated stem cells were isolated from aspirated whole rat BM using ficoll and cultured in α-MEM complete growth medium in 10 cm petridish. After two days, adherent cells after washing twice in petridish were added α-MEM growth medium and then mesenchymal cells were characterized using CD105 marker in third passage and labeled PKH26. Then haemopoietic stem cells (HSCs) were isolated with magnetic beads CD34+ and differentiated in vitro, and then induced monocytes with LPS. Animal experiment used 28 male Wistar rats, and divided them into 4 groups. After transplantation combined, both cells between monocyte derived HSc (mHSCs) and rat BM-MSC were analyzed expression of pair box gen 4 (Pax4), pancreatic and duodenal homeobox (Pdx1), C-peptide using immunohistochemistry, then secretion of insulin and C-peptide analyzed using indirect ELISA. Results showed that the expressions of Pax4, Pdx1, C-peptide found in the surface membrane cell of pancreatic cell, and secreted C-peptide and insulin were shown significant (P < 0.05) in transplanted group 2, 3 and 4, but in group 3 were transplanted with combined cells more dominant than non-combined cells. Conclusions suggested that combining of induced monocytes-derived HSCs and rat BM-MSCs has accelerated homing MSCs into injured pancreatic tissue.

Item Type: Article
Uncontrolled Keywords: Induced Monocyte Derived HSCs, Rat BM-MSCs, Homing, Injured Pancreas
Subjects: R Medicine > R Medicine (General)
R Medicine > RD Surgery > RD701-811 Orthopedic surgery
Divisions: 01. Fakultas Kedokteran > Orthopaedi & Trauma
Creators:
CreatorsNIM/NIDN
Fedik Abdul Rantam, NIDN. 0010035907UNSPECIFIED
Purwati, NIDN. 0025027203UNSPECIFIED
Budi SetiawanUNSPECIFIED
Sony Wibisono, NIDN. 8827800016UNSPECIFIED
Ferdiansyah Mahyudin, NIDN. 8890010016ferdiansyah@fk.unair.ac.id
Joni Wahyuhadi, NIDN. 8885900016joniwahyuhadi@fk.unair.ac.id
Edward MouliUNSPECIFIED
Dwikora Novembri Utomo, NIDN. 8897700016dwikora-novembri-u@fk.unair.ac.id
Heri Suroto, NIDN. 8802630017UNSPECIFIED
Candra BumiUNSPECIFIED
Depositing User: hanif kurniawan
Date Deposited: 07 Nov 2019 04:21
Last Modified: 07 Nov 2019 04:21
URI: http://repository.unair.ac.id/id/eprint/89762
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