Early Graft Tunnel Healing After Anterior Cruciate Ligament Reconstruction With Intratunnel Injection of Bone Marrow Mesenchymal Stem Cells and Vascular Endothelial Growth Factor

Rosy Setiawati, NIDN. 0015027604 and Dwikora Novembri Utomo, NIDN. 8897700016 and Fedik Abdul Rantam, NIDN. 0010035907 and Nadia Natassia Ifran and Nicolaas C. Budhiparama (2017) Early Graft Tunnel Healing After Anterior Cruciate Ligament Reconstruction With Intratunnel Injection of Bone Marrow Mesenchymal Stem Cells and Vascular Endothelial Growth Factor. Orthopaedic Journal of Sports Medicine, 05 (06). 01-08. ISSN 23259671

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Official URL: https://journals.sagepub.com/doi/abs/10.1177/23259...

Abstract

Background: Bone marrow mesenchymal stem cells (BM-MSCs) are multipotent adult stem cells and have become an important source of cells for engineering tissue repair and cell therapy. Vascular endothelial growth factor (VEGF) promotes angiogenesis and contributes fibrous integration between tendon and bone during the early postoperative stage. Both MSCs and VEGF can stimulate cell proliferation, differentiation, and matrix deposition by enhancing angiogenesis and osteogenesis of the graft in the tunnel. Hypothesis: Injection of intratunnel BM-MSCs and VEGF enhances the early healing process of a tendon graft. Study Design: Controlled laboratory study. Methods: In this controlled animal laboratory study, each of 4 groups of rabbits underwent unilateral anterior cruciate ligament (ACL) reconstruction with use of the ipsilateral semitendinosus tendon. The rabbits received intratunnel injection of BM-MSCs and VEGF with a fibrin glue seal covering the distal tunnel at the articular site. Evaluation using magnetic resonance imaging (MRI), collagen type III expression, and biomechanical analyses were performed at 3- and 6-week intervals. Results: All parameters using MRI, collagen type III expression, and biomechanical analysis of pullout strength of the graft showed that application of intratunnel BM-MSCs and VEGF enhanced tendon-to-bone healing after ACL reconstruction. Conclusion: Intratunnel injections of BM-MSCs and VEGF after ACL reconstruction enhanced graft tunnel healing. Overall, the femoral tunnel that received BM-MSCs and VEGF had better advanced healing with increased collagen type III fibers and better outcomes on MRI and biomechanical analysis. MRI is the most reliable tool for clinical use in evaluating stages of ACL healing after reconstruction, since biopsy is an invasive procedure.

Item Type: Article
Uncontrolled Keywords: graft tunnel healing, bone marrow mesenchymal stem cell, vascular endothelial growth factor, anterior cruciate ligament
Subjects: R Medicine > R Medicine (General)
R Medicine > RD Surgery > RD701-811 Orthopedic surgery
Divisions: 01. Fakultas Kedokteran > Orthopaedi & Trauma
Creators:
CreatorsNIM/NIDN
Rosy Setiawati, NIDN. 0015027604UNSPECIFIED
Dwikora Novembri Utomo, NIDN. 8897700016dwikora-novembri-u@fk.unair.ac.id
Fedik Abdul Rantam, NIDN. 0010035907UNSPECIFIED
Nadia Natassia IfranUNSPECIFIED
Nicolaas C. BudhiparamaUNSPECIFIED
Depositing User: hanif kurniawan
Date Deposited: 07 Nov 2019 04:31
Last Modified: 07 Nov 2019 04:31
URI: http://repository.unair.ac.id/id/eprint/89830
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