In Vitro Comparative Study of Osteogenic Differentiation Ability between Adipose and Bone Marrow Mesenchymal Stem Cell Applied to Bovine Demineralized Bone Matrix

Ferdiansyah Mahyudin, NIDN. 8890010016 and Dwikora Novembri Utomo, NIDN. 8897700016 and Mouli Edward, NIDN. 8829130017 and Lukas Widhiyanto, NIDN. 0023077404 and Christopher Antony Simanjuntak and Hudaya Nikmatullah (2018) In Vitro Comparative Study of Osteogenic Differentiation Ability between Adipose and Bone Marrow Mesenchymal Stem Cell Applied to Bovine Demineralized Bone Matrix. Journal of Biomimetics, Biomaterials and Biomedical Engineering, 38. pp. 59-66. ISSN 2296-9845

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Official URL: https://www.scientific.net/JBBBE.38.59

Abstract

Ideal bone graft must possess the desirable trait such as osteoconductive, osteoinductive and osteogenesis. Demineralized Bone Matrix (DBM) provides both osteoconductive and osteoinductive trait. Referring to the tissue engineering principle, the addition of mesenchymal stem cell would add the osteogenic trait to this procedure. The design of this study is experimental using Bovine DBM. Bone Marrow Mesenchymal Stem Cell (BMSCs) and Adipose Mesenchymal Stem Cells (ASCs) were taken from New Zealand white rabbit. There are two groups of treatment, divided into DBM implanted with BMSCs and DBM implanted with ASCs. Each BMSCs and ASCs groups is incubated in the normal and osteogenic culture plate. Evaluation is performed by counting the osteoblast and immunohistochemistry stain using Alkaline Phosphate and Osteocalcin. After 4 weeks of incubation, we found that the osteoblast count in BMSCs groups is higher compared to the ASCs groups in both culture condition (p<0.01) along with Alkaline Phosphate staining (p<0.05), while the Osteocalcin staining showed insignificant differences (p>0.05). This study revealed that xenogenic bovine DBM can act as the potential osteoinductive scaffold for the MSCs to differentiate. The tissue engineering application by combining MSCs and Bovine DBM can be considered as an alternative in managing bone defect cases.

Item Type: Article
Uncontrolled Keywords: Bone Defect, Demineralized Bone Matrix, Mesenchymal Stem Cell
Subjects: R Medicine > R Medicine (General)
R Medicine > RD Surgery > RD701-811 Orthopedic surgery
Divisions: 01. Fakultas Kedokteran > Orthopaedi & Trauma
Creators:
CreatorsNIM/NIDN
Ferdiansyah Mahyudin, NIDN. 8890010016ferdiansyah@fk.unair.ac.id
Dwikora Novembri Utomo, NIDN. 8897700016dwikora-novembri-u@fk.unair.ac.id
Mouli Edward, NIDN. 8829130017UNSPECIFIED
Lukas Widhiyanto, NIDN. 0023077404UNSPECIFIED
Christopher Antony SimanjuntakUNSPECIFIED
Hudaya NikmatullahUNSPECIFIED
Depositing User: hanif kurniawan
Date Deposited: 07 Nov 2019 08:11
Last Modified: 07 Nov 2019 08:11
URI: http://repository.unair.ac.id/id/eprint/89877
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