PENGARUH VANADIL SULFAT TERHADAP AKTIVITAS ENZIM TELOMERASE PADA PROLIFERASI SEL BETA PANKREAS MENCIT (Mus musculus) DENGAN DIABETES MELLITUS

MOHD SYAMIR MOHAMAD SHUKERI, 050710027 (2012) PENGARUH VANADIL SULFAT TERHADAP AKTIVITAS ENZIM TELOMERASE PADA PROLIFERASI SEL BETA PANKREAS MENCIT (Mus musculus) DENGAN DIABETES MELLITUS. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Aim: To investigate the influence of vanadyl sulfate on telomerase activities in pancreatic β cells proliferation of streptozotocin-induced diabetic mice.Methods: 25 mice were divided into five groups i.e. placebo, diabetic and treatment groups with three doses of vanadyl sulphate (5, 30 and 100 mg/kg BW).Diabetic mice model was induced twice by intraperitoneal administration of streptozotocin; 100 mg/kg on day 1 followed by 50 mg/kg on day 14.Administration of vanadyl sulphate in three doses was given on day 21 to day 28.The pancreas tissues was taken on day 28; stained with aldehide fuchsin for morphological quantitative analysis also immunohistochemical examination to observe the existence of telomerase activity. Results: Diabetic condition occurred on day 21 after streptozotocin injection and glucose levels increased significantly (p=0.015). In pancreatic islets of the diabetic group, damaged islets, decline in islet size, and degenerative effects on pancreas β cells were observed in comparison to the normal group. Administration of three different doses of vanadyl sulfate for 7 days significantly reduces blood glucose concentration (p<0.001). After treatment with vanadyl sulfate, pancreatic islets of Langerhans in the diabetic group showed a higher number of prominent β cells, intact islets and an increase in islet size compared to the diabetic group. The proliferative effect in diabetic mice treated with vanadyl sulfate showed a significant increase. This is shown by an increase in diameter of the islets (p<0.001) and the number of β cells on a single islet (p<0.001). Immunohistochemical examination shows that vanadyl sulphate is able to stimulate activation of telomerase. The telomerase activation causes a proliferative effect on pancreas β cells and is shown by islets morphology resembling close proximity to normal mice islet morphology visually. Conclusion: According to the histochemical and immunohisto -chemical results, it was concluded that vanadyl sulfate can significantly decrease blood glucose concentration and stimulate activation of telomerases that enhance β cell proliferation in experimental diabetes mice compared to normal mice.

Item Type: Thesis (Skripsi)
Additional Information: KKB KK2 FF 392 11 Shu p
Uncontrolled Keywords: DIABETES MELLITUS
Subjects: R Medicine
R Medicine > RC Internal medicine
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsEmail
MOHD SYAMIR MOHAMAD SHUKERI, 050710027UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorJunaidi Khotib, S.Si., M.Kes., Ph.D., Apt.UNSPECIFIED
Depositing User: Mr Mudjiono Mudj
Date Deposited: 06 Feb 2012 12:00
Last Modified: 15 Aug 2016 07:14
URI: http://repository.unair.ac.id/id/eprint/9010
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