LAILYA NISSA’US SHOLIHAH, 051511133209 (2019) AKTIVITAS ASETILKOLINESTERASE INHIBITOR DARI SUBFRAKSI n-BUTANOL SPONS Niphates olemda IN VITRO. Skripsi thesis, Universitas Airlangga.

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Abstract

Marine sponge has been reported as the source of many metabolites with various pharmacological activity, including acetylcholinesterase inhibitory activity. Previous study reported that methanolic extract of Niphates olemda sponge had acetylcholinesterase inhibitory activity with IC50 value of 56.78 μg/mL. Then the methanolic extract of N. olemda was partitioned using n-hexane, ethyl acetate and n-butanol. The results showed that the n-butanol fraction had a strong activity and the inhibitory precentage of fraction was 96.92% at a concentration of 100 μg/mL (Suciati et al., 2018). The aim of this study is to investigate the acetylcholinesterase inhibitory activity of n-butanol subfractions of N. olemda as well as to investigate of the secondary metabolites in the n-butanol subfractions obtained. Subfractionation of the n-butanol fraction of N. olemda was performed by using Vacuum Liquid Chromatography (VLC). The result of the acetylcholinesterase inhibitor activity test showed that the n-butanol subfractions from N. olemda had a low inhibition against AChE enzyme. Subfraction with the highest inhibition was subfraction 11 of 30.3 ± 6.8%. The compound identification was conducted by Thin Layer Chromatography (TLC) with anisaldehid H2SO4 stain visualization. The result showed purple colour indicate the presence of terpenoid compound.

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