AKTIVITAS ANTIHEPATITIS C DARI KOMBINASI KSTRAK ETANOL DAUN Ruta angustifolia L. DENGAN OBAT ANTIVIRUS HEPATITIS C SECARA IN VITRO

HUMAIROH MAHFUD, 051511133137 (2019) AKTIVITAS ANTIHEPATITIS C DARI KOMBINASI KSTRAK ETANOL DAUN Ruta angustifolia L. DENGAN OBAT ANTIVIRUS HEPATITIS C SECARA IN VITRO. Skripsi thesis, Universitas Airlangga.

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Abstract

Ruta angustifolia L. is a plant of rutaceae’s family which has been traditionally used forjaundice. Previous study showed that ethanol extract of R. angustifolia inhibit hepatitis C virus against JFH1a virus on hepatocyte cell Huh7 with IC50 3.0 ± 1.4 μg/ml. In order to continue our study, ethanol extract of R. angustifolia combined with antihepatitis C virus drugs such as simeprevir, telaprevir, and ribavirin to determine the activities and synergistic or additive effects caused by these combinations. The results of the combination activity of extract R. angustifolia with simeprevir obtained the increasing of antihepatitis C virus activity which is showeda decreasing IC50 simeprevir, which is from 43.84 ± 0.96 nM (single treatment) to 19.70 ± 0.28 nM (combination) with a combination index value (CI) = 0.833. The combination of extract R. angustifolia with telaprevir decreased IC50 telaprevir from 10.48 ± 0.11 nM (single treatment) to 3.64 ± 0.07 nM (combination) and CI value = 0.677. Then a combination of extract R. angustifolia with ribavirin which can decrease IC50 ribavirin from 10.43 ± 0.18μg/ml (single treatment) to 2.80 ± 0.03 μg/ml (combination) with CI value = 0.691. The Compusyn software possess a CI value <1, which means the three combinations have a synergistic effect. Toxicity analysis showed that the three combinations were not toxic to hepatocyte cells with percent viability cells higher than 90%. These results indicate that R. angustifoliahas the potential to be used as a recommendation for combination therapy for antihepatitis C virus.

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF.FT 18/19 Mah a
Uncontrolled Keywords: R. angustifoliaextract, combination therapy, and synergistic effect.
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi > Farmakognosi Fitokimia
Creators:
CreatorsNIM/NIDN
HUMAIROH MAHFUD, 051511133137UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorTutik Sri Wahyuni, S.Si., M.Si., Ph.D., Apt., '0025107704UNSPECIFIED
Depositing User: Dwi Marina
Date Deposited: 13 Nov 2019 04:02
Last Modified: 13 Nov 2019 04:02
URI: http://repository.unair.ac.id/id/eprint/90756
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