PENETAPAN KADAR ASAM BETULINAT DALAM KULTUR KALUS Solanum mammosum CGS

LOVELY QUR`AN ILMIAH, 051511133155 (2019) PENETAPAN KADAR ASAM BETULINAT DALAM KULTUR KALUS Solanum mammosum CGS. Skripsi thesis, Universitas Airlangga.

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Abstract

Determination of betulinic acid in callus culture Solanum mammosum CGS has been done. Thin-layer chromatographic method has been developed and validated for the analysis of betulinic acid in in callus culture S. mammosum CGS. Separation factor of betulinic acid (Rs = 1.85) was obtained by using mobile phase of hexane:ethyl acetate:acetic acid (7:3:0.03, v/v) (Rf 0.40) on silica gel 60F254 aluminium plates and densitometric determination was carried out after derivatization with anisaldehyde-sulphuric acid reagent at λ 540 nm. Linierity test showed the linier relationship between betulinic acid concentration and area (y = 3847.86x+1673.65 ; r = 0.99176). The proposed method was valid and successfully applied for determination of betulinic acid in callus culture S. mammosum CGS . The content of betulinic acid in the sampels were found around 0.76% w/w in simplicia.

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF.FT 26/19 Ilm p
Uncontrolled Keywords: Betulinic acid, thin layer chromatography-densitometri, callus culture Solanum mammosum CGS
Subjects: R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi > Farmakognosi Fitokimia
Creators:
CreatorsNIM/NIDN
LOVELY QUR`AN ILMIAH, 051511133155UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorSuciati S.Si., M.Phil., Ph.D., Apt.,, NIDN :'0004117905UNSPECIFIED
Depositing User: Dwi Marina
Date Deposited: 13 Nov 2019 04:43
Last Modified: 13 Nov 2019 04:43
URI: http://repository.unair.ac.id/id/eprint/90770
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