Role of Allogenic NK Cells Treatment in the Early Phase of Apoptosis on Poorly Differentiated Retinoblastoma Cells Culture

Hendrian Dwikoloso Soebagjo, NIDN. 8860900016 and Fitria Kusumastuti and Parma Ratna Jaya and Susy Fatmariyanti, NIDN. 8873800016 and Endang Retnowati and Siprianus Ugroseno Yudho Bintoro, NIDN. 8807700016 (2018) Role of Allogenic NK Cells Treatment in the Early Phase of Apoptosis on Poorly Differentiated Retinoblastoma Cells Culture. In: Proceedings of the International Meeting on Regenerative Medicine. SCITEPREES-Science and Technology Publications, Lda., Surabaya, pp. 194-201. ISBN 978-989--758-334-6

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Abstract

Introduction: Retinoblastoma is a retinal malignant tumor because of mutations on chromosome 13q14. This is associated with apoptosis deregulation processes. Both autologous or allogeneic NK cells play their roles in both innate and adaptive immune systems. They can stimulate apoptosis through several mechanisms. Methods: This study determined the role of allogeneic NK cells in retinoblastoma cell apoptosis. Poorly differentiated retinoblastoma tissues were tested with SDS-PAGE. Allogeneic NK cells were isolated from peripheral blood of the healthy family members of patients. There were two culture groups consisting of retinoblastoma cells only as the control and retinoblastoma treated with allogeneic NK cells as the treated group. Each group consisted of 10 well plates. Examinations of the expression of Bcl-2, Caspase-3, and Apoptosis were performed by the flowcytometry method. Result: The SDS PAGE electrophoresis tests showed strong expression of proteins with molecular weights of 1 4, 19, 28, 35, 53 and 85 kDa. The percentage of early-phase apoptosis is higher than late phase. There were significant correlations (α <0.05) between Bcl-2, Caspase-3, and the ratio of both on cell apoptosis. Conclusion: In conclusion, allogeneic NK cells play a role in retinoblastoma cell apoptosis, especially in the early phase. It is expected to be one of the new strategies in cancer immunotherapy

Item Type: Book Section
Uncontrolled Keywords: Poorly differentiated retinoblastoma, NK cells, allogeneic, apoptosis, cancer immunotherapy
Subjects: R Medicine > R Medicine (General)
R Medicine > RE Ophthalmology
Divisions: 01. Fakultas Kedokteran > Ilmu Penyakit Mata
Creators:
CreatorsNIM/NIDN
Hendrian Dwikoloso Soebagjo, NIDN. 8860900016hendrian.dwikoloso@fk.unair.ac.id
Fitria KusumastutiUNSPECIFIED
Parma Ratna JayaUNSPECIFIED
Susy Fatmariyanti, NIDN. 8873800016UNSPECIFIED
Endang RetnowatiUNSPECIFIED
Siprianus Ugroseno Yudho Bintoro, NIDN. 8807700016UNSPECIFIED
Depositing User: hanif kurniawan
Date Deposited: 18 Nov 2019 01:27
Last Modified: 18 Nov 2019 01:27
URI: http://repository.unair.ac.id/id/eprint/91042
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