ANALISIS KUALITATIF DAN KUANTITATIF P-AMINOFENOL DALAM SEDIAAN SIRUP PARASETAMOL DENGAN METODE KCKT

ASTRID KUSUMA PUTRI, 050810239 (2012) ANALISIS KUALITATIF DAN KUANTITATIF P-AMINOFENOL DALAM SEDIAAN SIRUP PARASETAMOL DENGAN METODE KCKT. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

A simple isocratic reversed-phase HPLC method was developed and validated for the determination of p-aminophenol, the major degradation impurity of paracetamol, in pharmaceutical syrups. The analytes were separated on a RP-18 LiChrospher® column (250 mm x 4.00 mm i.d., 5 μm particle size). A mobile phase of MeOH and 0.01M phosphate buffer pH 4.07 (26:74 v/v) at flow rate of 1 ml/min, with oven temperature set at 30°C was suitable for the separation and determination of p-aminophenol in simulation paracetamol syrups. The UV detection was carried out at 275.8 nm. The chromatographic parameters such as retention times, capacity factor, tailing factor, number of theoretical plates, %RSD of peak area and resolution factors were determined. The developed method was found to be linear over concentration ranges of 1.01 – 6.06 μg/ml for p-aminophenol (r2 = 0.9996, Vxo = 1.48%). The limit of detection (LOD) and limit of quantification (LOQ) for p-aminophenol were 0.18 μg/ml and 0.54 μg/ml, respectively. Validation acceptance criteria were met in all cases. Then the developed method was successfully applied to determine p-aminophenol in three generic paracetamol syrups in Indonesia, resulting that p-aminofenol were not detected in sample.

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF.KF.02/13 Put a
Uncontrolled Keywords: PARACETAMOL, P-AMINOPHENOL, HPLC, SYRUP
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RM Therapeutics. Pharmacology > RM300-666 Drugs and their actions
Divisions: 05. Fakultas Farmasi > Farmastika
Creators:
CreatorsNIM/NIDN
ASTRID KUSUMA PUTRI, 050810239UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorM. Yuwono, Prof. Dr. rer. nat. H., MS., Apt.UNSPECIFIED
Depositing User: mat sjafi'i
Date Deposited: 18 Feb 2013 12:00
Last Modified: 05 Sep 2016 02:37
URI: http://repository.unair.ac.id/id/eprint/9131
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