INE KARNI, 061714453001 (2019) KOMPARASI GEN pfhaB1 Pasteurella multocida TIPE B ISOLAT LOKAL SAPI DAN KERBAU ASAL NUSA TENGGARA TIMUR (NTT) PENELITIAN EKSPLORATIF LABORATORIS. Thesis thesis, Universitas Airlangga.

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Abstract

Almost all regions in East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Septicaemia Epizootica (SE), it is caused by Pasteurella multocida (P. multocida) Serotypes B: 2. The pfhaB1 gene is the virulence factor of P. multocida. The objectives of this study were analysis the phylogenetic tree, homology and prediction B cell epitope of the gene a coding pfhA protein of isolates P. multocida from Buffalo and Cattle NTT. The isolates P. multocida were recultured and followed by microscopic and biochemical tested, PCR, sequencing, homology, phylogenetic tree, and also prediction of B cell epitope. The PCR result showed pfhaB1 gene have DNA band 506 bp. The isolates P. multocida from Buffalo NTT and Cattle NTT are the same cluster and they have phylogenetic relatedness closely with Katha strain vaccine and also isolates from Iran and India. The isolate P. multocida from buffalo have four identical epitopes with Katha strain vaccine, but isolate from cattle have two identical epitopes with Katha strain vaccine. Between buffalo NTT, cattle NTT and Katha strain vaccine have two identical epitopes. Base on log score the epitope with NGYLLNELLQEL structure peptides from buffalo isolate has the most immunogenic epitope caused it has highest log score.

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