PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium) Repository

PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium)

NURITA TRI WIJAYANTI, NIM01151801603 (2019) PENGARUH FIBRIN GLUE DAN MEDIA TERKONDISI SEL PUNCA MESENKIMAL LIMBUS TERHADAP EKSPRESI COLLAGEN-1 (Col-1), MATRIX METALLOPROTEINASE-3 (MMP-3), DAN TISSUE INHIBITOR OF METALLOPROTEINASE-2(TIMP-2) PADA SEL FIBROBLAS TENON SEBAGAI MODEL PENYEMBUHAN LUKA PASCATRABEKULEKTOMI IN VITRO (Studi Eksperimental Laboratorium). Thesis thesis, Universitas Airlangga.

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Official URL: http://lib.unair.ac.id

Abstract

Purpose : investigate antifibrotic effect of FG and LMSCs-CM on expression of Col-1, MMP-3, and TIMP-2 in HTFs of glaucomatous eye. Methods : HTF’s from glaucomatous eyes divided into 4 groups consist of FBS 2% control group, MMC (Mitomycin C), Fibrin Glue (FG) and LMSCs-CM treated group. The experiment was conducted in 7 days. Col-1, MMP-3, and TIMP-2 expression were assessed with immunofluorescence staining. Intensity of fluorescence was measured using ImageJ software. Difference of expression level among groups were analyzed using Oneway ANOVA test followed by post-hoc test with p<0.05 considered to be significant Results : Col-1 expression were decreased FG (0.78 ± 0.09 pixels) and LMSCs- CM conditioned media (0.78 ± 0.10 pixels) compared to the FBS 2% control group (1.52 ± 0.33 pixels) (p = 0,000). However, MMC provides better antibrotic effects on HTFs revealed with decreased of Col-1 expresion in MMC group (0.41 ± 0.28 pixels). MMP-3 expression levels were found to be increased in the MMC group (1.87 ± 0.58 pixels), FG (1.67 ± 0.48 pixels), and LMSCs-CM (1.58 ± 0.56 pixels) compared to the FBS 2% control group (0.90 ± 0.11 pixels) (p = 0.002). A decrease in the expression of TIMP-2 showed significant results in the MMC group (0.72 ± 0.31 pixel), FG (0.73 ± 0.40 pixel) and LMSCs-CM (0.80 ± 0.23 pixel) compared to the PBS control group of 2% (1.36 ± 0.36 pixels) (p = 0.002). Conclusion : FG and and LMSCs-CM might have antifibrotic effect on HTFs through modulation of extracellular matrix

Item Type:

Thesis (Thesis)

Additional Information:

KKA KK PPDS.IKM. 15-19 Wij p

Uncontrolled Keywords:

fibrin glue, limbal mesenchymal stem cell, antifibrotic, mitomycin c

Subjects:

R Medicine > RE Ophthalmology

Divisions:

01. Fakultas Kedokteran > Ilmu Penyakit Mata

Creators:

Creators	NIM
NURITA TRI WIJAYANTI, NIM01151801603	NIM01151801603

Contributors:

Contribution	Name	NIDN / NIDK
Thesis advisor	EVELYN KOMARATIH, NIDN: '8830900016	UNSPECIFIED
Thesis advisor	Fedik Abdul Rantam, NIDN: '0010035907	UNSPECIFIED

Depositing User:

Tatik Poedjijarti

Date Deposited:

11 Dec 2019 02:42

Last Modified:

11 Dec 2019 02:42

URI:

http://repository.unair.ac.id/id/eprint/92470

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