Mufasirin and Wayan T. Artama and Sumartono and Sri Hartati (2015) Cloning of cDNA Encoding Membrane Protein of Tachyzoite of Toxoplasma gondii In pUC19. Prosiding 1st International Seminar on "Natural Resources Biotechnology : From Local to Global". pp. 349-359.

	Text (Prosiding) Bukti 32 Cloning of cDNA Encoding Membrane .....pdf Download (1MB)
	Text (Peer Review) Peer review C 32.pdf Download (2MB)

Abstract

The aim of the research is to clone cDNA encoding membrane protein of Toxoplasma gondii using pUC19 for developing molecular diagnosis and vaccines. Total RNA and messenger RNA were isolated from tachyzoites of Toxoplasma gondii that growing up in Swiss strain mice using PolyAtract®mRNA Isolation Systems (Promega) and synthesis of cDNA using Riboclone®cDNA Synthesis Systems (Promega). Complementary DNA was ligated with EcoRI adaptor using Riboclone®EcoRIAdaptor Systems (Promega). preparation pUC19 vector, pUC19 ligated with cDNA was transformed in E. Coli DH5α. Plasmid recombinant was analyzed using endonuclease restriction enzyme (EcoRI and HindIII), total protein isolation and immunoblotting. Results of transformation using the pUC19 vector obtained two recombinant clones carrying genes encoding membrane proteins

Actions (login required)

View Item