LAYLA HIDAYATUS S., 050710187 (2011) VALIDASI METODE PENETAPAN KADAR VITAMIN LARUT LEMAK DAN VITAMIN LARUT AIR SECARA SIMULTAN DENGAN METODE KCKT. Skripsi thesis, UNIVERSITAS AIRLANGGA.

Preview	Text (ABSTRAK) gdlhub-gdl-s1-2012-hidayatuss-22341-ff2791-k.pdf Download (336kB) | Preview
	Text (FULLTEXT) gdlhub-gdl-s1-2012-hidayatuss-18683-ff2791-v.pdf Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Fat- and water- soluble vitamins were separated on ACE C18 column (150mm x 4,6 mm, 3 μm particle size) in a single run using combined isocratic and linear gradien elution with a mobile phase consisting of 0,010% trifluoroacetic acid of pH 3,9 (solvent A) and methanol (solvent B). A linear gradient profile (A:B) started at 88:12 at the flow rate 0,5 ml min -1 and held for the first 4 mins, then decreased up to 2:98 at the flow rate 0,6 ml min -1 during the next 7 mins, held for next 6 mins, increased up to 0:100 at the flow rate 0,7 ml min -1 in 2 mins, and held on until a total analysis time of 29 min and finally linearly increased up to 88:12 to reach initial conditions. The most suitable wevelength for simultanious vitamin determination was 280 nm. The method was applied for the emultion sample of pharmaceutical preparation (multi-vitamin). The result were in good agreement with the declared values.

Actions (login required)

View Item