IFAH YULISTYANI, 050710106 (2011) KARAKTERISASI ANTIBIOTIK Streptomyces G ISOLAT TANAH RUMAH KOMPOS BRATANG SURABAYA. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Cases of infection because the microorganisms are still common in Indonesia. Therefore, the use of antibiotics is still occupying the high percentage in the use of drugs. Streptomyces G has a relatively strong antimicrobial activity. Accordingly, investigating the potential of Streptomyces G as a resource for new antibiotic are needed. Contact bioautography technique has used to investigate the biological activity of Streptomyces G. Using this method, the active compounds were detected directly. The fermentation of Streptomyces G were extracted using ethyl acetate. Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739 were used as test bacteria in bioautography. The ethyl acetate fractions of Streptomyces G were deposited as spots on silica gel plates and developed in a toluene:ethanol mixture (8:2). The plates were then subjected to contact bioautography, which showed inhibition zone against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739 indicating the presence of antibacterial components. On the thin layer chromatograms, the extracts were separated into one spot by viewing under ultraviolet at 254 nm (Rf value 0.54) and two spots (Rf value 0.53 and 0.64) at 365 nm. However, only one (Rf value 0.53) showed antibacterial activity. Active component was found as heat-stable. MIC value of Streptomyces G extracts were 128,75 ppm against Escherichia coli ATCC 8739 and 2801,6 ppm against Staphylococcus aureus ATCC 6538.

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