Lilik Herawati and Kristanti Wanito Wigati and Wahyu Wibowo and Harjanto Joso Muljono (2016) Survival Rate Of Animal Subject To Interval And Continuous Addition Of High Calorie Diet. In: International Symphosium on Global Physiology and 25th Indonesian Physiological Society (IPS/IAIFI) Annual Meeting. Gadjah Mada University Press, Yogyakarta, pp. 42-48. ISBN 978-602-386-237-5

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Abstract

High calorie diet increases the risk of diabetes mellitus. Nowadays, teens tend to consume high calorie diet. However, mechanisms of the effect of high calorie diet are still poorly understood, therefor animal model are needed to help to resolve it. This research was aimed to develop animal model that able to survive from continuous and interval administration of high calorie diet. Eight week of age of Balb/c mice were used as the subject. High calorie diet was dietary standard and an additional 8gram/kgBW and 14gram/kgBW of glucose solution via oral galvage, which were given continuousl (7 times/week) and by interval of once, twice, and 3 times a week. Ad libitum 0.05gram/cc of glucose solution was given for all groups based on their continous or interval treatment. The subjects’ survival rate were observed for 8 weeks. The survival rate of all the interval groups showed no significant difference with the control group. The group with continuous feeding of 14gram/kgBW showed significant difference with the control group (p=0.008) and the survival rate was 16.7%. The group with continuous feeding of 8gr/kgBW showed no significant difference with the control group (p=0.468), but it was prone to have worse survival rate (36.4%). This study revealed that survival rate of animal subjects with the interval addition of high calorie diet can be used as the animal model. As for continuous feeding, further investigation is needed to establish the appropriate dose for better survival rate.

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