JALU SATWIKO SULANINDYO KATON, 050911102 (2013) ANALISIS KCKT TERHADAP EKSTRAK ETANOL 80% DAN FRAKSI-FRAKSI DARI HERBA Ruta angustifolia (L.) Pers. Skripsi thesis, UNIVERSITAS AIRLANGGA.

Preview	Text (ABSTRAK) ABSTRAK.pdf Download (204kB) | Preview
	Text (FULLTEXT) FULLTEXT.pdf Restricted to Registered users only Download (2MB) | Request a copy

Abstract

One of some herbs that is potentially developed as a new drug for antihepatitis C is Ruta angustifolia (L.) Pers. It is commonly known as “inggu” in Indonesia and has been traditionally used for jaundice remedies. The 96% ethanol extract of this herbs has been also confirmed in the previous study for its inhibiting activity against hepatitis C viral replication, with IC50 value 2.23 μg/ml. In order to develop this herb as antihepatitis C phytopharmaceutical product, a control quality is required to ensure the compounds within the herb stay steady from batch to batch. The aim of this study is to acquire the chromatogram profile of 80% ethanol extract and its fractions of Ruta angustifolia herbs using HPLC instrument in order to be used as a standard for quality control purpose. This study comprises selecting a suitable analytical wavelength, mobile phase, selectivity test, precision test, purity test and a similarity between analyte UV spectrum and standard UV spectrum. The HPLC instrument that is used for this study is Shimadzu LC-6 series with a RP column Shim-pack Shimadzu (4.6 x 250 mm), meanwhile the mobile phase conditions are acetonitrile – water (20:80 v/v), eluted isocratically, flow rate 0.6 ml/minute and analyzed at wavelength 255 nm for 120 minutes. A rutin compound has been detected on 80% ethanol extract and butanol fraction in Rt min 16.425; 16.469; and 16.737 for 80% ethanol extract, and 16.936; 16.371; and 16.392 for butanol fraction. Those rutins, both in 80% ethanol extract and butanol fraction, have a similar spectrum with standard rutin, with match factor > 0.9500, hence chromatogram profile of 80% ethanol extract and butanol fraction of Ruta angustifolia herbs can be used to standardize, with rutin as its standard. Regarding the precision test, 80% ethanol extract and all of its fractions meet the criteria (≤ 5%).

Actions (login required)

View Item