VALIDASI METODE KLT- DENSITOMETRI UNTUK PENETAPAN KADAR KOLKISIN DALAM SEDIAAN HERBAL KERING KEMBANG SUNGSANG (Gloriosa superba Linn)

RICKY DESRIANTO, 050911277 (2013) VALIDASI METODE KLT- DENSITOMETRI UNTUK PENETAPAN KADAR KOLKISIN DALAM SEDIAAN HERBAL KERING KEMBANG SUNGSANG (Gloriosa superba Linn). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The purpose of this study was to validate the TLC-densitometry method for the determination of colchicine in the preparation of dried herbs Gloriosa superba L. include selectivity, linearity, limit of detection, limit of quantitation, accuracy and precision. In addition, make the determination of levels of colchicine in Gloriosa superba dried herbal preparations L. Selectivity trials using a mobile phase of chloroform: diethylamine (9:1) and the wavelength selected for analysis was 356 nm colchicine. Linearity test result in the regression equation y = 24308 x + 795.9 with a correlation coefficient (r) = 0.9989 and VXO = 3.999%. Test the limits of detection and quantitation limits produce regression equation y = 42722.4912x + 47.1530 with a correlation coefficient (r) = 0.9988. Obtained from the calculation of the price of 2.7 ng detection limit and quantitation limit of 8.9 ng. Accuracy test is done using standard addition, yield 88.37% recovery% - 119.3%, with an average of 100.8%. Precision test methods of generating price correlation coefficient (KV) of 10.22%. Determination of colchicine in 2,5032 g of dried herbal preparations Gloriosa superba L. obtained an average grade of 27,07 μg colchicine, to meet the therapeutic dose of 0.5 mg colchicine needed 46,1845 g of dried herbal preparations Gloriosa superba L.

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF.KF 42/13 Des v
Uncontrolled Keywords: CONTENT COLCHICINE
Subjects: Q Science > Q Science (General) > Q1-390 Science (General)
Q Science > QD Chemistry > QD1-999 Chemistry
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM/NIDN
RICKY DESRIANTO, 050911277UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorDr. Djoko Agus Purwanto, Apt., M.Si, Dr., Apt., M.SiUNSPECIFIED
Depositing User: shiefti dyah alyusi
Date Deposited: 08 Jan 2014 12:00
Last Modified: 10 Aug 2016 08:47
URI: http://repository.unair.ac.id/id/eprint/9734
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