VALIDASI METODE KROMATOGRAFI LAPIS TIPIS DENSITOMETRI UNTUK PENETAPAN KADAR KOLKISIN DALAM INFUS DAUN Gloriosa superba Linn.

AULIYA HILMI, 050911169 (2013) VALIDASI METODE KROMATOGRAFI LAPIS TIPIS DENSITOMETRI UNTUK PENETAPAN KADAR KOLKISIN DALAM INFUS DAUN Gloriosa superba Linn. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Validation of thin layer chromatography-densitometry for determination of colchinine in Gloriosa superba Linn. infus has been done. The purpose of this study was to obtain a valid thin layer chromatography densitometry for determination colchicine in Gloriosa superba Linn. infus using selectivity/ specificity, accuracy, precision and linearity parameter. The results were as follows, selective mobile phase obtained was chloroform:diethylamine (9:1). Linearity test showed that there were linear relationship between colchicine concentration and area with coefficient correlation (r = 0,9965) and Vxo = 8,19%. The average recovery was 85,77 ± 6,39 %, (RSD = 7,45%). RSD for instrument precission was 3,74%. The results of this study indicate that this method was valid and fulfill requirement of AOAC, with selectivity for colchicine (Rs = 2, 14 and 3, 5), linearity (r=0,9965), accuracy (85,77%)

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF.KF 45/13 Hil v
Uncontrolled Keywords: CHROMATOGRAPHY
Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM/NIDN
AULIYA HILMI, 050911169UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorProf. Dr. Sudjarwo, Apt., MS, Prof. Dr., Apt., MSUNSPECIFIED
Depositing User: shiefti dyah alyusi
Date Deposited: 08 Jan 2014 12:00
Last Modified: 10 Aug 2016 09:36
URI: http://repository.unair.ac.id/id/eprint/9737
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