Michael Golden Kurniawan (2020) Ekspresi Mmp-1 Dan Densitas Kolagen Tipe 1 Pada Sel Pulpa Tikus Setelah Aplikasi Kombinasi Ekstrak Propolis Dan Kalsium Hidroksida. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background: Calcium hydroxide, the golden standart for pulp capping treatment was known to have some disadavantages such as high solubility and formation of tunnel defect. Propolis with its anti-inflammatory properties is expected to improve the treatment outcome when it is combined with calcium hydroxide as direct pulp capping agent. Objective: Evaluating of the effect of calcium hydroxide and Propolis extract combination as a direct pulp capping agent based on its capability to form reparative dentin bridge which is represented by expression of MMP1 and Density of Collagen Type 1 in perforated rat teeth model. Material and Methods: This experimental study using a total of 30 maxillary first molar of wistar rat which then randomly divided into experimental combination of calcium hydroxide and Propolis extract group, calcium hydroxide group, and control group. The cavities then sealed with Cention. After 3 and 7 days, rats then sacrificed from each group, and sections of the teeth were obtained. After being decalcified, specimens underwent histological evaluation under light microscope to identify the presence of odontoblast-like cell, inflammation cells, and dentin bridge. The immunohistochemistry (IHC) method using anti-MMP1 and anti-Collagen Type 1 was then performed to evaluate the expression of MMP1 and Collagen Type 1 density. The results then statistically evaluated by Kolmogorrov Smirnov, homogeneity, and one way ANNOVA tests. Results: Combination of calcium hydroxide and Propolis extract group shows most cells expressing Collagen type 1 density and shows least cells expressing MMP1. Conclusion: MMP1 expression after aplication of propolis extract combined with calcium hydroxide was smaller and collagen type 1 density after aplication of propolis extract combined with calcium hydroxide was higer than application with calcium hydroxide after 3 and 7 day.

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