In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm

Edy Setiti Wida Utami and Sucipto Hariyanto (2019) In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm. Scientifica, 2019 (810513). pp. 1-6. ISSN 2090908X

[img] Image (Abstract)
C03. Abstract.pdf

Download (3MB)
[img] Text (Fulltext)
C03. Full_In Vitro Seed.pdf

Download (3MB)
[img] Text (Review dan Validasi)
C03. Review dan Validasi.pdf

Download (3MB)
[img] Text (Similarity)
C03. Similarity_In Vitro Seed.pdf

Download (2MB)
Official URL: https://www.hindawi.com/journals/scientifica/2019/...

Abstract

Phalaenopsis amboinensis, an epiphytic orchid, has a great potential for commercial exploitation in the cut-flower industry. It is difficult to propagate vegetatively as it naturally grows slowly. ,erefore, there is a need to improve the propagation methods to avoid endangering its natural populations. ,e objective of this study was to identify the best medium and organic supplements for seed germination and plantlets development of P. amboinensis. Seeds from 4-month-old hand-pollinated orchids were sown on different strengths of MS and VW culture media: Murashige and Skoog (MS), 1/2 MS, Vacin and Went (VW), and 1/2 VW. Optimum seed germination, i.e., 90.7%, was achieved on VW medium. VW medium was suitable for seedling formation and allowed 51.4% of seedling development from protocorm within 10 weeks of culture. When 15% (v/v) coconut water was added together with banana homogenate (10 g·L −1) to the VW medium, the plantlets grew to the highest length and had the highest dry weight (62.1 mm and 15.5 g, respectively). ,e roots and leaves of the plantlets grew vigorously in this medium. Plants regenerated via in vitro seed germination processes were successfully acclimatized in greenhouse conditions, and the survival rate was more than 85%.

Item Type: Article
Uncontrolled Keywords: Germination; Seed
Subjects: Q Science > Q Science (General)
Q Science > QK Botany
Divisions: 08. Fakultas Sains dan Teknologi > Biologi
Creators:
CreatorsNIM
Edy Setiti Wida UtamiUNSPECIFIED
Sucipto HariyantoNIDN0009025604
Depositing User: Mr Vega Andi Budiman
Date Deposited: 28 Aug 2020 12:22
Last Modified: 14 Sep 2020 07:18
URI: http://repository.unair.ac.id/id/eprint/97846
Sosial Share:

Actions (login required)

View Item View Item