Septa Devi Adetya Putri and Isnaeni and M. Faris Adrianto (2019) Antibacterial Activities of Extract n-Butanol-Methanol (1:1) Filtrat of the Fermentation Results of Streptomyces sp. B10 Against Mycobacterium tuberculosis H37Rv: An Experimental Study. Indian Journal of Public Health Research and Development, 10 (9). pp. 1511-1516. ISSN 0976-0245

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Abstract

Background: Streptomycessp. is one of the sources of first choice antibiotic for tuberculosis treatment. n-butanol is the best solvent to extract antibacterial compound from fermented Streptomycessp. isolates. Aim: To analyze anti-tuberculosis activity of extract n-butanol-methanol (1:1) filtrate of the fermentation results of Streptomyces sp. B10 against Mycobacterium tuberculosis H37Rv. Method: This was a laboratory experimental study. 3 comparisons of the type of solution were observed once a week for 3 weeks. Antibacterial activity test was analyzed from morphological observations on Ziehl-Neelsen staining, then minimum inhibitory concentration and minimum bactericidal concentration were determined. Result: Bacterial growth began in the second week starting from a concentration of 10,50 ppm to a concentration of 1,31 ppm but a concentration of 43.000 ppm to a concentration of 5.375 ppm didn’t show any bacterial growth. The results of Ziehl-Neelsen staining show a red and rod-shaped Mycobacterium tuberculosis H37Rv colonies at a concentration of 2.687,5 ppm to a concentration of 1,31 ppm and at a positive control. A concentration of 2.687,5 ppm was determined as minimum inhibitory concentration and a concentration of 5.375 ppm was determined as minimum bactericidal concentration. Conclusion: Extract n-butanol-methanol (1:1) filtrate of the fermentation results of Streptomyces sp. B10 at a concentration of 2.687,5 ppm to 5.375 ppm has antibacterial activity against Mycobacterium tuberculosis H37Rv. This extract can be used as an antibiotic formula for tuberculosis.

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