Paulus Sugianto and Abdulloh Machin and Hanik Badriyah Hidayati and Mohammad Hasan Machfoed (2013) Ekspresi Kaspase 8 dan Perubahan Jumlah Sel Neuroglia pada Regulasi Mekanisme Apoposis Sel Otak Akibat Keracunan Merkuri. Neurona, 30 (2). pp. 1-5. ISSN 0216-6402

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Abstract

Introduction : Mercury is a potent neurotoxin since it induces apoptosis and inflammatory respond in brain. Caspases e.g. caspase 8is one of central effectors of apoptosis and serve as signaling mediators that orchestrate apoptotic execution pathways by cleaving a subset of cellular proteins. Whereas brain inflammatory response is triggered by the activation of neuroglial e.g. microglial cells and astrocytes and this glial reactivity has been used as an early marker of neurotoxicity.To study the effects of neurotoxicity mercury we use caspase 8 and microglial as a parameter of methyl mercury neurotoxicity. Aims : To observe the process of apoptosis and inflammatory processes in the brain caused by methylmercury poisoning rats Rattus novergicus. Methods : Rattus novergicus aged 4 months was used as experiental model and devided into 3 group of treatment with orally administered: aquabidest; methylmercury 0,2 mg and 0,4 mg/BW/day. Brains of the mice were removed after 30 days of exposure and tissue samples were obtained for examination. Caspase8 was identified by imunohystochemistry staining using Avidin-biotin complex method. Microglial cell was counted from hystophatology examination of the HE stained brain tissue. Results : Mean of caspase 8 in group of aquabidest; methylmercury 0.2 and 0.4 mg/BW/day were 68?8.95;197.3?36.83;275.6?34.19 respectively whereas mean of microglial cell count:65.6?19.61;137.1?15.32;167.9?12.8 respectively. Conclusions : Mercury exposure increases both of caspase 8 expression and neuroglia cell count in Rattus novergicus brain tissue. The result suggests that methylmercury induces apoptosis and inflammatory respond in brain.

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