Dewiyani Indah Widasari and Yoshihiko Yano and Didik Setyo Heriyanto and Takako Utsumi and Laura Navika Yamani and Hanggoro Tri Rinonce and Widya Wasityastuti and Maria Inge Lusida and Soetjipto and Rina Okada and Yoshiki Murakami and Toshihito Tanahashi and Takeshi Azuma and Yoshitake Hayashi (2014) A Deep-Sequencing Method Detects Drug-Resistant Mutations in the Hepatitis B Virus in Indonesians. Intervirology, 57. pp. 384-392. ISSN 03005526

	Text (Artikel) A Deep-Sequencing Method Detects Drug-Resistant Mutation in the Hepatitis B Virus in Indonesia.pdf Download (600kB)
	Text (Peer Review) A Deep Sequencing.pdf Download (1MB)
	Text (Similarity) A Deep-Sequencing Method Detects Drug-Resistant Mutation In The Hepatitis B Virus In Indonesia.pdf Download (2MB)

Abstract

Objective: The long-term administration of a nucleos(t)ide analogue (NA) for the treatment of chronic hepatitis B may encourage the emergence of viral mutations associated with drug resistance. Minor populations of viruses may exist before treatment, but are difficult to detect because of technological limitations. Identifying minor viral quasispecies should be useful in the clinical management of hepatitis B virus (HBV) infection. Methods: Six treatment-naïve Indonesian patients with chronic HBV infection participated in this study. The polymerase region of the HBV genome, including regions with known drug-resistant mutations, was subjected to capillary sequencing and MiSeq sequencing (Illumina). Mutations were analyzed with Genomics Workbench software version 6.0.1 (CLC bio). Results: The mean mapping reads for the six samples was 745,654, and the mean number of amplified fragments ranged from 17,926 to 25,336 DNA reads. Several known drug-resistant mutations in the reverse transcriptase region were identified in all patients, although the frequencies were low (0.12-1.06%). The proportions of the total number of reads containing mutations I169L/M, S202R, M204I/L or N236S were >1.0%. Conclusion: Several known NA-resistant mutations were detected in treatment-naïve patients in Indonesia using deep sequencing. Careful management of such patients is essential to prevent drug-resistant mutations from spreading to other patients.

Actions (login required)

View Item