Dalia A. Sabry (2020) Comparative study about aging effect on retina and cerebellum of Columba livia domestica. Acta Zoologica.

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Abstract

The present research explores the ageing impact of Columba livia domestica on retina and cerebellum. It is an available and a widespread bird species. Three ageing groups (n = 5) of male Columba were collected at about three weeks, six months and five years old. Histological studies were made. Morphometric measurement of both retinal and cerebellar cortex layer thicknesses has been determined. For evaluation of the neuronal synapses, quantitative GFAP biochemically and immunohistochemically staining was performed. Also, biochemical analysis of glucose transporter 1, serotonin and dopamine was carried out to demonstrate their functional activity. Flow cytometric assessments of quantitative cell death were accomplished by using annexin V. The present findings revealed quantitative and qualitative increase of GFAP parallel with decrease of serotonin, dopamine and glucose transporter 1 as well as increase of UR and LR annexin V reflecting cell death in old groups. These were confirmed by damaging of the retina and cerebellar layers. The present findings concluded that retinal and cerebellar cells associated with ageing were determined by sharp rise in both cell death and GFAP levels. Additionally, a decrease of dopamine, serotonin and GluT1 level manifested maybe led to functional alteration of both retina and brain.

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