Jola Rahmahani, - and Salamah, - and Mufasirin, - and Wiwiek Tyasningsih, - and Mustofa Helmi Effendi, - (2020) Antimicrobial Resistance Profile of Escherichia Coli from Cloacal Swab of Domestic Chicken in Surabaya Traditional Market. Biochemical and Cellular Archives, 20 (1). pp. 2993-2997. ISSN 0972-5075, E ISSN 0976-1772

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Abstract

The aim of this research is to determine antimicrobial resistance’s profile of Escherichia coli from cloacal swab of domestic chicken in Surabaya traditional market to several antibiotic such as aztreonam, ciprofloxacin, gentamicin, chloramphenicol, dan tetracycline. Sixty samples were teken from Pucang, Keputran, and Wonokromo traditional market.Those ampleswere isolated and identified using Mac Conkey Agar (MC Agar), Eosin Methylen Blue Agar (EMB Agar), Gram staining, IMViC and Triple Sugar Iron Agar (TSI Agar). The results shows that 58 out of 60 of the sample was identified as Escherichia coli. The isolate of Escherichia coli then further tested to sensitivity test using Kirby-Bauer method. Final results indicates that 48% of samples were resistant to tetracycline, 38% resistance to ciprofloxacin, 16% resistance to chloramphenicol, 10% resistance to gentamicin and 7% resistance to aztreonam.The results of this study illustrate the potential of domestic chickens as a reservoir for the spread of antibiotic resistance to the community Key words : Domestic chicken, cloacal swabs, antibiotic resistance, Escherichia coli

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