Nenny Harijani and Ayu Wandari and Mustofa Helmi Effendi and Wiwiek Tyasningsih (2020) Molecular Detection of Encoding Enterotoxin C Gene and Profile of Antibiotic Resistant on Staphylococcus Aureus Isolated from Several Dairy Farms in East Java, Indonesia. Biochemical and Cellular Archives, 20 (1). pp. 3081-3085. ISSN 0972-5075, E ISSN 0976-1772

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Abstract

The purpose of this study were to identify the encoding gene for enterotoxin C and study the profile of antibiotic resistanton Staphylococcus aureus from raw milk in East Java, Indonesia. Raw milk samples of 148 samples obtained from four dairy farms. Bacterial identification was based on the growth in Mannitol Salt Agar (MSA) and Gram staining and catalase & coagulase tests. 24(16.2%) out of 148 milk samples were for positive Staphylococcus aureus isolation. Antibiotic sensitivity testing using Cefoxitin, Penicillin, Amphicillin, Erythromycin and Tetracyclin antibiotics showed 1(4%), 17(71%), 16(67%), 24 (100%) and 8(33%) isolates were resistant to the antibiotics, respectively. MRSA isolate showed that 1 isolate was positive by using Cefoxitin disc diffusion test. The molecular identification on enterotoxin C gene by PCR showed that 2(8%) isolates were positive contain enterotoxin C gene. It was concluded that the raw milk can be a potential reservoir forStaphylococcus aureus strains to threat public health Key words : Staphylococcus aureus, antibiotic sensitivity test, MRSA, enterotoxin C gene

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