Venri Novryantoro (2020) Karakterisasi Biologis Virus Newcastle Disease Isolat Lapang Burung Dara (Columba livia). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aims of this research was to determine the biological characterization of viruses from pigeon isolates (Columba livia) through pathogenicity tests including Mean Death Time (MDT), Intracerebral Pathogenicity Index (ICPI), and Intravenous Pathogenicity Index (IVPI). The research sample was collected from necropsy organs of 20 pigeons taken from several regions in East Java and one positive control LaSota (ATCC). Samples were isolated on the embryonated chicken eggs and identified through the HA Test then confirmed by the HI Test. Positive samples were tested for MDT with a dilution of 10-1-10-18 dose 0.1 ml and observed for 8 days. The fresh ND virus suspension then conducted an ICPI test dose of 0.05 ml, observed for 8 days and IVPI test dose of 0.1 ml, observed for 10 days. The MDT values of isolates ND/B1/2019, ND/B2/2019, ND/B3/2019, and ND/BG1/2019 respectively were 91.2 hours, 112.8 hours, 110.4 hours, and 124.8 hour. The ICPI value of ND/B1/2019 isolate was 0.2375, ND/B2/2019 isolate was 0.375, ND/B3/2019 isolate was 0.5375, and ND/ BG1/2019 isolate was 0.3. The IVPI value of ND/B1/2019 isolate was 0, ND/B2/2019 isolate was 0, ND/B3/2019 isolate was 0, and ND/BG1/2019 isolate was 0. It was concluded that samples of ND/B1/2019, ND/B2/2019, ND/B3/2019, and ND/BG1/2019 were declared lentogenic pathotypes based on MDT, ICPI, and IVPI tests.

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