Udijanto Tedjosasongko and Katsuyuki Kozai (2017) Level and Strain Number of Streptococcus Mutans in Toddlers After The Initial Acquisition. In: TIMNAS7-JSMID4. FKG UNAIR, Surabaya, pp. 234-238. ISBN 978-989-758-341-4

	Text (NASKAH) 38. Udijanto Prosiding ( Level and Strain Number of ... ).pdf Download (736kB)
	Text (SIMILARITY) 39...Level and Strain Number of Streptococcus Mutans in Toddlers after The Initial Acquisition-1.pdf Download (2MB)
	Text (VALIDASI) 39... Udijanto 4 Prosiding (Level and Streain...).pdf Download (833kB)

Abstract

Streptococcus mutans (S mutans) are known as major bacteria in human dental caries. Previous experiments reported that S mutans could transmit between persons. The purpose of this study were to determine the level and number of strain type of S mutans after the initial acquisition in children. Samples were the plaque of the 39 children in range of age 0-5 years old of a day nursery in Hiroshima City Japan which were taken with toothbrush every month for 30 months. The S mutans isolated were taken by inoculation using mitis salivarius agar and BHI broth respevtively. Identification was done by mannitol, sorbitol fermentation test and insoluble glucan synthesis test. The strain types of S mutans were determined by comparing the chromosomal DNA fingerprint of S mutans using restriction endonuclease EcoRI and Haell. Twenty-one children (54%) acquired S mutans and 18 children (46%) remain free. The level of S mutans were increased gradually and changed (more).

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