Arrokhman, S. and Wijayanti, N. and Agoes Soegianto (2017) Survival and osmoregulation of juvenile of hybrid grouper (Epinephelus fuscoguttatus � Epinephelus lanceolatus) during acclimation in calcium-supplemented freshwater. Aquaculture International, 25 (2). pp. 693-704.

Abstract

The aim of this research was to evaluate the effect of calcium concentration in water to the osmoregulation and survival of hybrid grouper (Epinephelus fuscoguttatus � Epinephelus lanceolatus) acclimated in freshwater environment. There was no mortality observed in all groups during acclimation to the freshwater up to 3 �. Once the fish reached the freshwater (0 �), rapid mortality was occurred. Lower salinity induced the lower serum osmolality. Lowest serum osmolality value was observed in the positive control group at 6 h post-transfer (HPT) to freshwater. The values of muscle water content were not significantly different among all groups in all salinities. The highest Na+�K+-ATPase (NKA) activities were observed in the 500 and 1000 mg/L CaCl2 groups at D10 1 and 6 HPT, respectively, whereas lowest NKA activities were recorded in the positive control and 100 mg/L CaCl2 groups at D10 6 HPT. This study shows that the hybrid grouper demonstrates excellent tolerance in environment up to 3 �. © 2016, Springer International Publishing Switzerland.

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