Retno Handajani and Mochammad Thaha and Mochammad Amin and Citrawati Dyah Kencono Wungu and Edhi Rianto and Pranawa (2016) Anti-HIV dan Subtipe HIV pada pasien Hemodialisis (Anti-HIV and HIV Subtype in Hemodialysis Patients). Indonesian Journal of Clinical Pathology and Medical Laboratory, 22 (2). pp. 182-186. ISSN 0854-4263

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Abstract

Anti-Human Immunodeficiency Virus (Anti-HIV) was performed from 100 plasma Chronic Kidney Disease (CKD) stage 5 patients with continuous hemodialysis (HD) at the Hemodialysis Instalation Dr Soetomo hospital, Surabaya, Indonesia, using three (3) kind of reagents: Tri-line HIV Rapid test Device from Acon for HIV 1/2/O as strips form, Foresight HIV 1/2/O Antibody EIA Test Kit from Acon and Anti-HIV 1+2/Subtype O ELISA from Axiom. HIV RNA and HIV subtype were detected by Reverse Transcription Polymerase Chain Reaction (RT-PCR) based on HIV gag region and analysis of DNA result. Seventy three % patients were hemodialysed twice in a week and only 14% with duration more than five (5) years. Most of the patients (43%) were hemodialysed between 100−300 times. From the 100 plasma samples was obtained only one (1%) man patient plasma sample with positive anti-HIV. A weak positive of RT-PCR result was not succeed to be sequenced for determining the HIV subtype. This cause was suspected due to low levels of HIV RNA in blood. The results of this study was expected can be used as an additional management consideration of hemodialysis patients at the Hemodialysis Unit.

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