Eko Widayanto and Nurwasis and Dicky Hermawan and Arief Fidianto and Paramita Putri and Yuyun Rindiastuti and Willy Sandhika and Igo Syaiful Ihsan and Hendrian Dwikoloso Soebagjo and Evelyn Komaratih (2020) Intracameral injection of limbal mesenchymal stem cells conditioned media (LMSCs-CM) improve clinical outcome with delayed on Na-K ATPase corneal endothelial pump recovery in phacoemulsified rabbit eyes. Biochemical and Cellular Archives, 19 (2). pp. 4889-4897. ISSN 09725075

	Text (Artikel) Intracameral Injection of limbal mesenchymal stem cells Conditioned media (LMSCs-CM) improve clinical outcome with delayed on Na-K ATPase Corneal Endothelial Pump Recovery in Phacoemulsified .pdf Download (208kB)
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Abstract

To investigate the effect of LMSCs-CM on central corneal thickness (CCT) and Na+/K+ ATPase of endothelial cells expression inphacoemulsifiedrabbit eyes. This was a true experimental laboratory study on rabbits using a pre and posttest control group design. Approximately 24 rabbit eyes were exposed to phacoemulsification ultrasound, then randomly divided into 2 groups. The control group was injected using 0.2 ml of physiological saline (BSS) intracamerally, while the treatment group was injected with 0.2 LMSCs-CM intracamerally. Central corneal thickness was evaluated on the 3rd day after treatment, followed by enucleation to analyzed Na+/K+ ATPase expression using immunohistochemistry.The LMSCs-CM significantly reduced corneal edema and inflammation resulting in clinical improvement. There was no mean difference of CCT between groups (p = 0.372, α>0.05). However, CCT was higher in the control group (62.00 ± 27.41) when compared to the treatment group (50.00 ± 36.45 um). Similarly, the expression of Na + / K + ATPase in both groups was not significantly different (p = 0.973, α>0.05). After 3 days, LMSCs-CM exerted clinical improvements, but had a delay in functional recovery, in regards to Na+/K+ ATPase expression in the corneal endothelial cells.

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