Rachma Ayuningtyas (2020) Optimasi Dan Validasi Metode Analisis Metabolit Nitrofuran Dalam Pangan Olahan Udang Secara Kckt-Uv/Pda. Thesis thesis, UNIVERSITAS AIRLANGGA.

	Text (HALAMAN DEPAN) 1. HALAMAN DEPAN.pdf Download (441kB)
	Text (ABSTRACT) 2. ABSTRACT.pdf Download (162kB)
	Text (BAB 1 PENDAHULUAN) 3. BAB 1 PENDAHULUAN.pdf Download (139kB)
	Text (BAB 2 TINJAUAN PUSTAKA) 4. BAB 2 TINJAUAN PUSTAKA.pdf Restricted to Registered users only until 19 February 2024. Download (266kB) | Request a copy
	Text (BAB 3 KERANGKA KONSEPTUAL) 5. BAB 3 KERANGKA KONSEPTUAL DAN HIPOTESIS.pdf Restricted to Registered users only until 19 February 2024. Download (131kB) | Request a copy
	Text (BAB 4 METODE PENELITIAN) 6. BAB 4 METODE PENELITIAN.pdf Restricted to Registered users only until 19 February 2024. Download (197kB) | Request a copy
	Text (BAB 5 HASIL PENELITIAN) 7. BAB 5 HASIL PENELITIAN.pdf Restricted to Registered users only until 19 February 2024. Download (279kB) | Request a copy
	Text (BAB 6 PEMBAHASAN) 8. BAB 6 PEMBAHASAN.pdf Restricted to Registered users only until 19 February 2024. Download (153kB) | Request a copy
	Text (BAB 7 KESIMPULAN DAN SARAN) 9. BAB 7 KESIMPULAN DAN SARAN.pdf Restricted to Registered users only until 19 February 2024. Download (93kB) | Request a copy
	Text (DAFTAR PUSTAKA) 10.DAFTAR PUSTAKA.pdf Download (141kB)
	Text (LAMPIRAN) 11. LAMPIRAN.pdf Restricted to Registered users only until 19 February 2024. Download (303kB) | Request a copy
	Text (PERNYATAAN KESEDIAAN PUBLIKASI) TF.04-21-Kesediaan Publikasi-Rachma A - Rachma Ayuningtyas.pdf Restricted to Registered users only Download (132kB) | Request a copy

Abstract

Furazolidone, furaltadone, and nitrofurantoin are nitrofuran antibiotics that are still illegally used as feed additives in shrimp farming. Due to their carcinogenicity, it is therefore needed to control the food products containing shrimps as the main ingredient. Nitrofuran are readily metabolized to the corresponding metabolites in the animal tissue. This study aims to validate the HPLC method for determining levels of nitrofuran metabolites in shrimp meatballs. The metabolites of furazolidon, furaltadone and nitrofurantoin i.e. 3-amino-2-oxazolidinone (AOZ), 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) and 1-aminohydantoin (AHD) were derivatized with 2-nitrobenzaldehyde, extracted into ethyl acetate, washed with hexane and applied to HPLC with UV-PDA detection. The separation was performed on C-18 column 46x250 mm, 5 μm using the mobile phase of 20 mM ammonium acetate and acetonitrile (70:30), with a flow rate of 0.5 ml/min and column temperature of 40ºC, injection volume of 100 μL. The method showed good separation of metabolites and other interferences with analysis time of 40 minutes, resulting good linear response in the concentration ranging from 50 to 100 ng/mL. The accuracy was found to be 79.82-103.99% for AOZ; 79.86-100.68% for AMOZ; and 89.70-103.62% for AHD; whereas the RSD for repeatability test was 6.12- 20.02%; 4.15-19.48%; and 3.30-14.38% for AOZ, AMOZ and AHD. LOD and LOQ obtained were between 3.09 and 10.29 ng/mL (AOZ); 6.84 and 22.82 ng/mL (AMOZ); and 4.61 and 15.36 ng/mL (AHD). The proposed method can be used as screening method for detection of nitrofuran metabolite contamination in shrimp meatballs.

Actions (login required)

View Item