Maria Apriliani Gani (2021) Studi Komparasi Osteokonduktivitas Natural dan Synthetic Hydroxyapatite Serta Keterlibatan Polaritas Makrofag pada Defek Tulang. Thesis thesis, UNIVERSITAS AIRLANGGA.

	Text (HALAMAN JUDUL) 1. HALAMAN JUDULL.pdf Download (249kB)
	Text (RINGKASAN) 2. RINGKASAN.pdf Download (122kB)
	Text (DAFTAR ISI) 3. DAFTAR ISI.pdf Download (122kB)
	Text (BAB 1) 4. BAB 1 PENDAHULUAN.pdf Download (118kB)
	Text (BAB 2) 5. BAB 2 TINJAUAN PUSTAKA.pdf Restricted to Registered users only until 7 April 2024. Download (307kB) | Request a copy
	Text (BAB 3) 6. BAB 3 KERANGKA KONSEPTUAL.pdf Restricted to Registered users only until 7 April 2024. Download (152kB) | Request a copy
	Text (BAB 4) 7. BAB 4 METODE PENELITIAN.pdf Restricted to Registered users only until 7 April 2024. Download (159kB) | Request a copy
	Text (BAB 5) 8. BAB 5 DATA DAN ANALISIS HASIL PENELITIAN.pdf Restricted to Registered users only until 7 April 2024. Download (358kB) | Request a copy
	Text (BAB 6) 9. BAB 6 PEMBAHASAN.pdf Restricted to Registered users only until 7 April 2024. Download (144kB) | Request a copy
	Text (BAB 7) 10. BAB 7 KESIMPULAN DAN SARAN.pdf Restricted to Registered users only until 7 April 2024. Download (59kB) | Request a copy
	Text (DAFTAR PUSTAKA) 11. DAFTAR PUSTAKA.pdf Download (199kB)
	Text (LAMPIRAN) 12. LAMPIRAN.pdf Restricted to Registered users only until 7 April 2024. Download (561kB) | Request a copy
	Text (PERMOHONAN EMBARGO) 13. PERMHONAN EMBARGO.pdf Restricted to Registered users only Download (731kB) | Request a copy

Abstract

Bone is an important organ that is the main component of the skeleton. Bone defect is a serious condition; if not treated properly, may cause delay or bone healing failure. This study aimed to determine the effect of macrophage polarity on the osteoconductivity of BHA-GEL and HA-GEL scaffold in vivo. The in vivo study model was carried out by implanting BHA-GEL and HA-GEL scaffold into the femoral defect of Wistar rats (defect diameter 2.2 mm, scaffold diameter 2 mm). The scaffold's characteristics in the form of morphology, pore size, and Ca/P ratio were examined using SEM-EDX. The compressive strength was measured using mini autograph. Osteoconductivity was examined based on histological results dan radiological images using X-rays, while macrophage polarity using immunohistochemical techniques. Immunohistochemical results showed that the BHA-GEL scaffold did not show signs of chronic inflammation as seen in the HAGEL scaffold group. Radiological data showed that the BHA-GEL scaffold caused acceleration of defect repair, in line with histological data, which showed accelerated migration of osteoclasts, osteoblasts, and osteocytes. The BHA-GEL scaffold has a round particle shape, while the HA-GEL scaffold has an irregular particle shape. The mean pore sizes of BHA-GEL and HA-GEL scaffold were 0.633 ± 0.089 and 0.361 ± 0.036 μm, respectively (p=0.035). The average compressive strength of the BHA-GEL and HA-GEL scaffolds was 61.714 ± 6.163 and 40.766 ± 4.513 MPa (p=0.041). Meanwhile, the Ca/P ratio of BHA-GEL and HA-GEL scaffold was 1.63 ± 0.05 and 1.44 ± 0.05, respectively (p<0.0001). Based on the study present it can be concluded that the BHA-GEL scaffold is osteoconductive and produces a beneficial immune response compared to the HA-GEL scaffold.

Actions (login required)

View Item